Verification of the Accuscan gold reader and RIDA smart phone application rapid test kits in detection and quantification of aflatoxin levels in maize from selected regions in Kenya

Quality Assurance and Safety of Crops & Foods Pub Date : 2022-10-13 DOI:10.15586/qas.v14i4.1118

Beatrice Nafula Tenge, William Maina Muiru, John Wangai Kimenju, C. Schwake-Anduschus, Samuel Linguya Kimaru, C. Nkonge

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引用次数: 7

Abstract

The study aimed to verify the effectiveness of two detection kits namely, Accuscan gold reader and RIDA total aflatoxin smart phone application in assessing levels of aflatoxins’ contamination in maize. Three regions of Kenya were selected namely, Kisumu, Makueni and Kilifi. Confirmatory tests were done using liquid chromatography tandem mass spectrometry (LC-MS/MS). The Accuscan gold reader showed mean aflatoxin levels of 14.52, 5.18, and 3.04 μg/kg and RIDA smart phone application had levels of 26.08, 26.68, and 4.05 μg/kg for Kilifi, Makueni, and Kisumu, respectively. The LC-MS/MS confirmed the presence of AFB1 and AFB2 with AFB1 of 97.5 μg/kg in Makueni and 76.2 μg/kg in Kilifi. Maize from Kilifi and Makueni was contaminated and unfit for human and animal consumption. Also, low rainfall, high temperature and high relative humidity directly increased the levels of aflatoxins, resulting in contamination. Therefore, the detection kits are recommended for use by maize farmers. Confirmatory tests should be done with maize having levels of aflatoxin above 10 μg/kg. Adequate drying and handling of maize and proper storage with good aeration should be ensured.

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验证了控告金阅读器和RIDA智能手机应用快速检测试剂盒对肯尼亚选定地区玉米中黄曲霉毒素水平的检测和定量

本研究旨在验证两种检测试剂盒(即控告金阅读器和RIDA总黄曲霉毒素智能手机应用程序)在评估玉米黄曲霉毒素污染水平中的有效性。肯尼亚的三个地区被选中，即基苏木、马库尼和基利菲。采用液相色谱串联质谱法(LC-MS/MS)进行验证试验。在智能手机应用软件RIDA中，Kilifi、Makueni和Kisumu的黄曲霉毒素平均含量分别为26.08、26.68和4.05 μg/kg。LC-MS/MS检测证实了AFB1和AFB2的存在，其中AFB1在Makueni为97.5 μg/kg，在Kilifi为76.2 μg/kg。Kilifi和Makueni的玉米受到污染，不适合人类和动物食用。此外，低降雨量、高温和高相对湿度直接增加了黄曲霉毒素的含量，导致污染。因此，建议玉米种植者使用该检测试剂盒。对黄曲霉毒素含量高于10 μg/kg的玉米应进行确认试验。应确保玉米的充分干燥和处理，并在通风良好的条件下妥善储存。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Quality Assurance and Safety of Crops & Foods

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