PEMBUATAN VIRGIN COCONUT OIL DENGAN EKSTRAK JAMUR Aspergillus niger SERTA UJI ANTIBAKTERI VCO DENGAN BAKTERI Staphylococcus aureus.

I. W. Suirta, I. A. R. A. Asih
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Abstract

ABSTRAK   Telah dilakukan pembuatan virgin coconut oil (VCO) dengan ekstrak jamur Aspergillus niger serta uji antibakteri VCO dengan bakteri Staphylococcus aureus. Kualitas VCO ditentukan dengan uji kadar air, angka asam, angka penyabunan, angka iod, uji organoleptik, dan analisis GC-MS. Ekstraksi VCO tanpa menggunakan ekstrak jamur hanya mendapatkan VCO sebanyak 5,859 g. Penambahan ekstrak jamur A. niger 0,5% b/v  menghasilkan VCO sebanyak 8,832 g, menunjukkan terjadi kenaikan yang sangat signifikan (p<0,05). Hasil uji kadar air, angka asam, angka penyabunan, dan angka iod masing-masing diperoleh: 0,1958; 0,2929; 5,0487; dan 0,2781, hasil ini sesuai dengan baku mutu VCO yang ditetapkan. Hasil uji organoleptik memberikan VCO yang tidak berwarna dan tidak berbau, dan hasil analisis GC-MS diperoleh kandungan asam lemak rantai medium dengan kandungan asam laurat sebagai komponen terbanyak. Hasil uji antibakteri terhadap bakteri Staphylococcus aureus menunjukkan kemampuan VCO dalam menghambat pertumbuhan bakteri dengan zona hambat 13,5 mm.   Kata kunci: antibakteri, Aspergillus niger, Staphylococcus aureus, virgin coconut oil.  ABSTRACT                    Preparation of virgin coconut oil (VCO) with Aspergillus niger fungi extract and the antibacterial test of the VCO with Staphylococcus aureus has been carried out. The quality test of the VCO included water content, acid number, saponification number, iodine value, organoleptic test, and GC-MS analysis. VCO extraction without using fungi extract only got 5.859 g of VCO. The addition of 0.5% w/v of A. niger fungi extracts produced 8.832 g of VCO, indicating a very significant increase (p <0.05). The water content, acid number, saponification number, and iodine value obtained were 0.158; 0.2929; 5.0487; and 0.2781 respectively, which met the VCO quality standard. The organoleptic test proved that the VCO was colourless and odourless. Meanwhile, the GC-MS analysis showed the content of medium-chain fatty acids with lauric acid as the largest component. The antibacterial test against Staphylococcus aureus indicated the ability of VCO to inhibit the growth of bacteria with an inhibition zone of 13.5 mm. Keywords: antibacterial, Aspergillus niger, Staphylococcus aureus, virgin coconut oil.
目前正在用一种niger曲霉提取物进行处女椰子油的抽象生产,并对一种葡萄球菌菌和一种VCO抗菌aureus。VCO的质量是由水能、酸性、凝结率、iod数、有机试验和GC-MS分析决定的。不使用蘑菇提取物提取VCO只获得5.859克的VCO。添加蘑菇提取物A. niger 0.5%的b/v产生8832 g的VCO,显示显著增加(p< 0.05)。检测结果:0.1958;0.2929;5.0487;0.2781,结果符合规定的VCO质量。有机微生物测试提供了无色无味的VCO,而GC-MS分析的结果是,GC-MS是含有海酸的介质链脂肪酸的主要成分。奥瑞斯葡萄球菌的抗菌试验结果显示,VCO在13.5毫米的消化区抑制细菌生长方面的能力。关键词:抗菌、尼日尔菌、葡萄球菌、维初椰子油。处女椰子油的禁止性真菌extract和VCO葡萄球菌菌试验已经被埋葬。VCO包括水的质量测试,acid number, saponification number,碘值值,有机分析和GC-MS分析。没有使用真菌extract的VCO只获得5859克的VCO。增加0.5%的w/v。水合的,acid编号,saponification number,碘值是0158;0。2929;5 . 0487;这符合VCO标准。VCO是无色和无味的。与此同时,gc女士分析(GC-MS analysis)揭示了中间链肥酸的结果,配以最大的对称性为基调。抗菌球菌试验限制了VCO抑制细菌生长的能力与13。5毫米抑制区。抗杆菌,尼日尔曲霉,葡萄球菌,处女椰子油。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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