Ilex paraguariensis: the effect of genotypes and growth phase on biomass, secondary metabolism and antioxidant activity of in vitro cultivated calli

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2022-01-01 DOI:10.37360/blacpma.22.21.4.33

R. L. Grunennvaldt, Juliana Degenhardt-Goldbach, J. Tomasi, F. Hansel, B. P. Brasileiro, P. Brooks, E. Gomes, U. Rossa, C. Deschamps

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Abstract

Yerba mate (Ilex paraguariensis) produces several secondary metabolites of interest to the pharmaceutical industry, such as chlorogenic acids and methylxanthines. These compounds have been produced in vitro by callus culture from different species. However, for I. paraguariensis, no studies upon the production of these compounds in vitro have been performed to date. In this work, we show that the concentration of secondary metabolites from I. paraguariensis callus is possible and highly dependent on the callus growth phase. We observed that the best phase for the production of secondary compounds in calli of yerba mate is the stationary growth phase on both genotypes tested. In this phase, higher levels of phenolic compounds, chlorogenic acid and 3,5-dicaffeoylquinic acid and greater antioxidant activity were observed. Chlorogenic acid and 3,5-dicaffeoylquinic acid presented positive correlation with antioxidant activity. For the first time, secondary compounds were reported in yerba mate calli cultivated in vitro.

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巴拉圭冬青:基因型和生长阶段对体外培养愈伤组织生物量、次生代谢和抗氧化活性的影响

巴拉圭茶(Ilex paraguariensis)产生一些制药工业感兴趣的次级代谢物，如绿原酸和甲基黄嘌呤。这些化合物是由不同种类的愈伤组织在体外培养中产生的。然而，对于巴拉圭刺槐，迄今为止还没有对这些化合物的体外生产进行过研究。在这项工作中，我们表明巴拉圭愈伤组织次生代谢物的浓度是可能的，并且高度依赖于愈伤组织的生长阶段。结果表明，两种基因型马黛茶愈伤组织产生次生化合物的最佳时期均为稳定生长期。在这一阶段，观察到更高水平的酚类化合物、绿原酸和3,5-二咖啡酰奎宁酸和更强的抗氧化活性。绿原酸和3,5-二咖啡酰奎宁酸与抗氧化活性呈正相关。首次报道了马黛茶愈伤组织中次生化合物的体外培养。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.