Validation and Development of Metformin obtained from the Extraction of Bougainvillea California Gold Flowers by UV Spectrophotometry, TLC and FTIR

Research and advances in pharmacy and life sciences Pub Date : 2022-08-02 DOI:10.46610/rapls.2022.v04i02.002

Sunitha N., P. Paul, M. a, Sravya D., S. P., Jaswanth V., L. Y., M. S

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Abstract

The project was considered to validate and develop an accurate, simple and precise, UV-Visible spectrophotometric, Thin Layer Chromatography (TLC) and Fourier transform Infrared Spectroscopy (FTIR) method for the estimation of Metformin present in extract of Bougainvillea California Gold flowers by using distilled water as solvent at a wavelength of 232 nm. The linearity of the metformin is 10-50 μg/ml with y = 0.0011x - 0.0006 as the regression equation r2 value was 0.9977. Recovery values for Metformin present in Bougainvillea California Gold flowers extract powder is found to 98.45% - 99.16%. The RSD % of intraday precision and intraday precision is 16.67 % and 61.53% respectively. LOQ and LOD values are 0.26 μg/ml and 0.0.08μg/mL respectively. The robustness %RSD values method was 64.38 – 64.66. Rf value of the extract was found to be 0.586 with 60:40 (methanol and water) in TLC. IR interpretation of both bougainvillea California gold flowers extract and Meformin were found to be similar. So the method was simple, precise, exact and economical. This method could be applicable for quantitative determination of the metformin in Bougainvillea California gold flowers extract.

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紫外分光光度法、薄层色谱法和红外光谱法对九重葛加州金花提取物二甲双胍含量的验证与研究

本项目旨在验证和建立一种准确、简单、精确的紫外可见分光光度法、薄层色谱法和傅里叶变换红外光谱法，以蒸馏水为溶剂，在232 nm波长下测定九重梅加利福尼亚金花提取物中二甲双胍的含量。二甲双胍的线性范围为10 ~ 50 μg/ml, y = 0.0011x ~ 0.0006，回归方程r2值为0.9977。三角梅加州金花提取物粉中二甲双胍的回收率为98.45% ~ 99.16%。日内精密度和日内精密度的RSD %分别为16.67%和61.53%。定量限为0.26 μg/ml，定量限为0.0.08μg/ ml。方法的稳健性%RSD值为64.38 ~ 64.66。在60:40(甲醇与水)的薄层色谱中，提取液的Rf值为0.586。三角梅加利福尼亚金花提取物和二甲双胍的红外光谱分析结果相似。该方法简便、精密度高、准确、经济。该方法可用于九重葛加州金花提取物中二甲双胍的定量测定。

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Research and advances in pharmacy and life sciences

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