Experimental study of intra-peritoneal instilation of recombinant human granulocyte colony-stimulating factor for the treatment of acute peritonitis

Abstract

Aim. In the experiment, the possibility of intraperitoneal application of recombinant human granulocyte colony-stimulating factor (G-CSF) for the treatment of acute peritonitis (AP) was investigated.Materials and methods. 60 non-linear white rats. AP was modeled by intra-abdominal puncture of 20% autofecal mixture. After 12 hours, a laparotomy and sanitation of the peritoneal cavity were performed. In 30 animals (control), a solution of decamethoxine was used. In the experiment, after sanitation, a solution of G-CSF on NaCl was injected into the peritoneal cavity at a dose of 0.1 million units per 100 g of mass. After 6, 12, 24 and 48 hours, a relaparotomy was performed and the parietal peritoneum was taken for examination. The number of cells was counted on digital copies of histological preparations.The results. After 6 hours: in the control, the number of polymorphonuclear leukocytes (PLL) - 2.7±0.39, in the experiment - 3.9±0.38 (р<0.05); in the control, the number of lymphocytes (LC) - 0.2±0.13, in the experiment - 0.7±0.33 (р<0.05). After 12 hours: in the control, the number of PLL - 3.1±0.62, in the experiment - 4.6±0.45 (р<0.05); in the control, the number of LC - 0-1 in the field of vision, in the experiment - 1.8±0.33; in the experiment, the number of fibroblasts (FB) was 0.9±0.27. After 24 hours: in the control, the number of PLL - 3.3±0.39, in the experiment - 1.3±0.33; in the control, the number of LC - 1.8±0.41, in the experiment - 2.3±0.33; in the control, the number of FB - 0.4±0.16, in the experiment - 1.6±0.31 (р<0.01); in the control, the number of macrophages (MF) - 0.4±0.16, in the experiment - 0.7±0.21; in the experiment, the number of plasma cells (PC) was 1.1±0.28. After 48 hours: in the control, the number of PLL - 2.1±0.27, in the experiment - 1.4±0.31 (р<0.05); in the control, the number of LC - 2.2±0.29, in the experiment - 3.3±0.37 (р<0.05); in the control, the number of FB - 1.9±0.34, in the experiment - 2.9±0.23 (р<0.05); in the control, the number of macrophages (MF) – 2.0±0.36, in the experiment – 3.4±0.22 (р<0.01); in the control, the number of PCs was 0.9±0.28, in the experiment - 3.1±0.27 (р<0.01). Conclusions. In animals with AP models, signs of a delay in the local response of immunocompetent cells for 12-24 hours, regeneration processes for 24-48 hours, and slowing down of the regression of the inflammatory process in the peritoneum are observed after the peritoneal cavity is cleaned with a decamethoxine solution. In animals that received intraperitoneal instillation of G-CSF after rehabilitation, signs of early activation of the local reaction of immune cells and regeneration processes are observed, along with the acceleration of the regression of inflammation. The results of the experiments indicate that intraperitoneal instillation of G-CSF can be used under clinical conditions for the treatment of patients with AP.