Genotoxic effects of boric acid and borax in zebrafish, Danio rerio using alkaline comet assay.

Nordisk psykologi Pub Date : 2015-07-30 eCollection Date: 2015-01-01 DOI:10.17179/excli2015-404

Nagihan Gülsoy, Cüneyd Yavas, Özal Mutlu

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Abstract

The present study is conducted to determine the potential mechanisms of Boron compounds, boric acid (BA) and borax (BX), on genotoxicity of zebrafish Danio rerio for 24, 48, 72 and 96-hours acute exposure (level:1, 4, 16, 64 mg/l BA and BX) in semi-static bioassay experiment. For that purpose, peripheral erythrocytes were drawn from caudal vein and Comet assay was applied to assess genotoxicity. Acute (96 hours) exposure and high concentrations of boric acid and borax increases % tail DNA and Olive tail moment. Genotoxicity was found for BA as concentration-dependent and BX as concentration and time dependent manner. In general, significant effects (P < 0,05) on both concentrations and exposure times were observed in experimental groups. DNA damage was highest at 96 h and 24 h for all BX and BA concentrations, respectively in peripheral blood of D. rerio. For the first time, our study demonstrates the effect of waterborne BA and BX exposure on genotoxicity at the molecular level, which may contribute to understanding the mechanism of boric acid and borax-induced genotoxicity in fish.

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利用碱性彗星试验研究硼酸和硼砂对斑马鱼（Danio rerio）的基因毒性影响。

本研究旨在通过半静态生物测定实验，确定硼化合物、硼酸（BA）和硼砂（BX）在斑马鱼急性暴露 24、48、72 和 96 小时（水平：1、4、16、64 毫克/升 BA 和 BX）后对其遗传毒性的潜在机制。为此，从尾静脉中抽取外周血红细胞，采用彗星试验来评估遗传毒性。急性（96 小时）接触高浓度的硼酸和硼砂会增加尾 DNA 和 Olive 尾矩的百分比。发现 BA 的遗传毒性与浓度有关，BX 的遗传毒性与浓度和时间有关。一般来说，实验组的浓度和暴露时间都有明显的影响（P < 0.05）。在所有浓度的 BX 和 BA 作用 96 小时和 24 小时后，D. rerio 外周血中的 DNA 损伤程度最高。我们的研究首次在分子水平上证明了接触水载 BA 和 BX 对遗传毒性的影响，这可能有助于了解硼酸和硼砂诱导鱼类遗传毒性的机理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nordisk psykologi

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