Autochthonous plant growth-promoting rhizobacteria enhance Thymus vulgaris growth in well-watered and drought-stressed conditions

IF 0.8 4区农林科学 Q3 AGRICULTURE, MULTIDISCIPLINARY

Zemdirbyste-agriculture Pub Date : 2021-12-06 DOI:10.13080/z-a.2021.108.044

D. Stamenov, S. Djuric, T. Hajnal-Jafari, S. Andjelkovič

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引用次数: 0

Abstract

The use of plant growth-promoting rhizobacteria (PGPR) in the production of medicinal plants is crucial, because agricultural chemicals are no longer acceptable in their production. This paper discusses the results of a recent study undertaken to isolate and characterize PGPR isolates of Bacillus, Pseudomonas and Azotobacter from common thyme (Thymus vulgaris L.) rhizospheric soil and monitor the effect of their application on seed germination and plant growth. The effect of selected isolates on seed germination and initial growth of common thyme was examined in controlled conditions. The impact of the isolates on the content of biochemical stress markers (total phenols and lipid peroxidation intensity) in germinated seeds was determined. The influence of the isolates on the plant growth under well-watered conditions (watered to 80% of water holding capacity (WHC)) and under water deficit stress (watered to 40% of WHC) was evaluated by measuring plant root and stem length in semi-controlled conditions. This study confirmed that indigenous rhizospheric bacteria of common thyme have multiple plant growthpromoting (PGP) properties. Among Bacillus 10 isolates, Pseudomonas 12 isolates and Azotobacter 9 isolates, only Pseudomonas isolate (P53) had all the tested PGP properties. The applied isolates had a positive effect on the examined plant growth parameters. The highest percentage of germination was determined in the treatment with P53 isolate (96%). The highest response for vigour index was observed with isolate A1 (3807%). All isolates, except B58 and B60, reduced stress by lowering the amount of both or at least one stress related molecules. The results of the experiment led to the conclusion that isolates Azotobacter sp. A1 and Pseudomonas sp. P53, both ACC-deaminase-producing bacteria, have the best effect on plant growth under well-watered and drought-stressed conditions, which indicates their potential to be used as a biofertiliser for promoting common thyme growth.

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原生植物促生根瘤菌在水分充足和干旱胁迫条件下促进寻常胸腺的生长

在药用植物的生产中使用促进植物生长的根瘤菌(PGPR)是至关重要的，因为农业化学品在药用植物的生产中已不再被接受。本文讨论了从普通百里香根际土壤中分离和鉴定芽孢杆菌、假单胞菌和固氮菌PGPR分离株的研究结果，并监测了它们对种子萌发和植物生长的影响。在控制条件下考察了所选菌株对普通百里香种子萌发和初始生长的影响。测定菌株对发芽种子生化胁迫标志物(总酚和脂质过氧化强度)含量的影响。在半控制条件下，通过测定植株的根和茎长，评价了各菌株在水分充足条件下(水分保持量80%)和水分亏缺胁迫下(水分保持量40%)对植株生长的影响。本研究证实了百里香根际原生细菌具有多种植物促生长(PGP)特性。在芽孢杆菌10分离株、假单胞菌12分离株和固氮杆菌9分离株中，只有假单胞菌(P53)具有全部PGP特性。施用的分离物对所测植物的生长参数有积极的影响。P53分离物处理的发芽率最高(96%)。A1分离物对活力指数的响应最高(3807%)。除B58和B60外，所有菌株都通过降低两种或至少一种与胁迫相关的分子的数量来降低胁迫。实验结果表明，产acc -脱氨酶的细菌Azotobacter sp. A1和Pseudomonas sp. P53在水分充足和干旱胁迫条件下对植物生长的影响最好，这表明它们有潜力作为促进百里香生长的生物肥料。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Zemdirbyste-agriculture AGRICULTURE, MULTIDISCIPLINARY-

CiteScore

1.80

自引率

11.10%

发文量

审稿时长

>12 weeks

期刊介绍： Zemdirbyste-Agriculture is a quarterly scientific journal which covers a wide range of topics in the field of agricultural sciences, agronomy. It publishes articles of original research findings in the English language in the field of agronomy (soil and crop management, crop production, plant protection, plant breeding and genetics, biotechnology, plant nutrition, agrochemistry, soil science, microbiology etc.) and related areas. Articles are peer-reviewed. Review, debating papers as well as those of a methodological nature will also be considered.