Comparative chromosome mapping of repetitive DNA in four minnow fishes (Cyprinidae, Cypriniformes)

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-09-21 DOI:10.36253/caryologia-1523

S. Aiumsumang, P. Chaiyasan, K. Khoomsab, W. Supiwong, A. Tanomtong, S. Phimphan

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引用次数: 0

Abstract

The present study focused on the repetitive DNA of the chromosome in four minnow fishes from the genera Danio Hamilton, 1822, Devario Heckel, 1843 and Rasbora Bleeker, 1859. Chromosomes were analysed using fluorescence in situ hybridization (FISH) with microsatellite probes including (CA)15, (CAC)10, (CGG)10, (GC)15 and (TA)15 staining. All species retained the diploid chromosome number 2n = 50 in male and female. The microsatellite sequences were mapped in the chromosomes of Danio albolineatus (Blyth, 1860), Devario regina (Fowler, 1934), Rasbora aurotaenia Tirant, 1885 and R. paviana Tirant, 1885. In most cases, the microsatellite was dispersed in the chromosome with conspicuous markings in the telomeric region and the whole genome, which suggests that sequences contribute to chromosome structure and may have played a role in the relationship of this fish group. The comparative genome mapping data presented here provide novel information on the structure and organisation of the repetitive DNA region of the minnow’s genome and contribute to a better understanding of the genomes of these minnows.

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四种鲦鱼(鲤科，鲤形目)重复DNA的比较染色体定位

本研究的重点是4种小鱼的染色体重复DNA，分别来自Danio Hamilton(1822)、Devario Heckel(1843)和Rasbora Bleeker(1859)。采用荧光原位杂交(FISH)技术对染色体进行分析，微卫星探针包括(CA)15、(CAC)10、(CGG)10、(GC)15和(TA)15染色。雌雄均保留二倍体染色体数目2n = 50。微卫星序列分别定位于Danio albolineatus (Blyth, 1860)、Devario regina (Fowler, 1934)、Rasbora aurotaenia Tirant(1885)和R. paviana Tirant(1885)的染色体上。在大多数情况下，微卫星分散在染色体上，在端粒区和整个基因组中有明显的标记，这表明序列有助于染色体结构，并可能在该鱼类群体的关系中发挥作用。本文提出的比较基因组图谱数据提供了关于鲦鱼基因组重复DNA区域的结构和组织的新信息，有助于更好地了解这些鲦鱼的基因组。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.