Oligoadenylate Synthetase Single-Nucleotide Polymorphisms Associated With Infection by Chikungunya Virus

IF 0.3 4区农林科学 Q4 ENTOMOLOGY

Southwestern Entomologist Pub Date : 2023-07-14 DOI:10.3958/059.048.0211

M. López-Rodríguez, I. Marino-Martínez, Á. Lugo‐Trampe, Sandra Caballero-Sosa, G. Trujillo-Rodríguez, María de Lourdes Ramírez Ahuja, Antonio Guzmán Velasco, Oscar Cienfuegos-Jiménez, M. Martinez-Fierro, A. A. Pérez-Maya, I. Delgado-Enciso, A. Flores-Suarez, I. Rodríguez-Sánchez, K. Trujillo-Murillo

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引用次数: 0

Abstract

Abstract. Chikungunya is a public health problem in Africa, Asia, and Latin America. Innate immune response (IIR) possesses enzymatic cleavage of nucleic acids of the agent as an important mechanism of viral counteraction. Oligoadenylate synthetase (OAS) enzymes are key regulators of RNase L to develop IIR against RNA viruses. OAS´ Single Nucleotide Polymorphisms (SNPs) have been related to susceptibility toward RNA-virus diseases. A cross-sectional study was done of 187 patients. OAS1 SNPs (rs1131454), OAS2 SNPs (rs1293762, rs15895, and rs1732778) and OAS3 SNPs (rs2285932 and rs2072136) genes were studied by qPCR in check and symptomatic patients to associate SNPs with susceptibility to disease. Relative risk (RR), Chi2, and Hardy-Weinberg equilibrium indicated p < 0.05 statistical significance. SNPs rs1131454 and rs1293762 showed statistically significant differences between cases and check. Homozygous genotypes GG of rs1131454 and CC of rs1293762 were considered risk factors (RR 1.59; 95% CI 1.19-2.14 and RR 1.91; 95% CI 1.31-2.78, respectively). Heterozygous genotypes GA of rs1131454 and AC of rs1293762 were protective factors (RR 0.67; 95% CI 0.48-0.94 and RR 0.56; 95% CI 0.37-0.83, respectively). Moreover, rs1293762 was statistically significant in allelic frequencies between cases and checks, with A allele a protective factor (RR = 0.55; 95% CI 0.39-.078) and C allele a susceptibility factor (RR = 1.80; 95% CI difference (p < 0.05) in the allelic frequencies between cases and checks, with A allele as protection factor (RR = 1.28-2.54). Our results add to previously reported evidence of susceptibility of certain populations with specific OAS-family SNPs against CHIKV.

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与基孔肯雅病毒感染相关的低聚腺苷酸合成酶单核苷酸多态性

摘要基孔肯雅热是非洲、亚洲和拉丁美洲的一个公共卫生问题。先天免疫反应(Innate immune response, IIR)是一种酶促因子核酸裂解的机制，是病毒对抗的重要机制。寡聚腺苷酸合成酶(OAS)酶是RNA酶L的关键调控因子，以产生针对RNA病毒的IIR。OAS的单核苷酸多态性(snp)与rna病毒疾病的易感性有关。对187例患者进行了横断面研究。采用qPCR方法研究OAS1 snp (rs1131454)、OAS2 snp (rs1293762、rs15895和rs1732778)和OAS3 snp (rs2285932和rs2072136)基因与疾病易感性的关系。相对危险度(RR)、Chi2、Hardy-Weinberg平衡p < 0.05有统计学意义。rs1131454和rs1293762的snp在病例与对照间差异有统计学意义。rs1131454的纯合子基因型GG和rs1293762的CC被认为是危险因素(RR 1.59;95% CI 1.19-2.14, RR 1.91;95% CI分别为1.31-2.78)。杂合基因型rs1131454的GA和rs1293762的AC是保护因子(RR 0.67;95% CI 0.48-0.94, RR 0.56;95% CI分别为0.37-0.83)。此外，rs1293762等位基因频率在病例和检查之间具有统计学意义，其中A等位基因是保护因子(RR = 0.55;95% CI 0.39- 0.078)和C等位基因为易感因子(RR = 1.80;以A等位基因为保护因子，病例与检查等位基因频率的95% CI差异(p < 0.05) (RR = 1.28 ~ 2.54)。我们的结果增加了先前报道的具有特定oas家族snp的某些人群对CHIKV易感性的证据。

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来源期刊

Southwestern Entomologist 生物-昆虫学

CiteScore

0.60

自引率

25.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Manuscripts submitted for consideration for publication in the Southwestern Entomologist should report results of entomological research in the southwestern United States or Mexico or should report results of studies on entomological species, relevant to this region, which may be done elsewhere, provided such results are geographically applicable. Manuscripts that report results of routine laboratory or field experiments for which the primary purpose is gathering baseline data or those that report results of a continuous evaluation program such as preliminary pesticide evaluation experiments, species lists with no supporting biological data, or preliminary plant resistance evaluations are not acceptable. However, reports of experiments with insecticides, acaricides, and microbials are acceptable if they are comprehensive and include data related to economics, resistance, toxicology, or other broad subject areas. Bibliographies will not be published in Southwestern Entomologist.