Circulating Extracellular Vesicle Tissue Factor Activity During Orthohantavirus Infection Is Associated With Intravascular Coagulation.

IF 1.6 3区生物学 Q4 EVOLUTIONARY BIOLOGY

Australian Systematic Botany Pub Date : 2020-09-14 DOI:10.1093/infdis/jiz597

Clare M Schmedes, Steven P Grover, Yohei M Hisada, Marco Goeijenbier, Johan Hultdin, Sofie Nilsson, Therese Thunberg, Clas Ahlm, Nigel Mackman, Anne-Marie Fors Connolly

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引用次数: 0

Abstract

Background: Puumala orthohantavirus (PUUV) causes hemorrhagic fever with renal syndrome (HFRS). Patients with HFRS have an activated coagulation system with increased risk of disseminated intravascular coagulation (DIC) and venous thromboembolism (VTE). The aim of the study was to determine whether circulating extracellular vesicle tissue factor (EVTF) activity levels associates with DIC and VTE (grouped as intravascular coagulation) in HFRS patients.

Methods: Longitudinal samples were collected from 88 HFRS patients. Patients were stratified into groups of those with intravascular coagulation (n = 27) and those who did not (n = 61). We measured levels of circulating EVTF activity, fibrinogen, activated partial prothrombin time, D-dimer, tissue plasminogen activator (tPA), plasminogen activator inhibitor 1 (PAI-1), and platelets.

Results: Plasma EVTF activity was transiently increased during HFRS. Levels of EVTF activity were significantly associated with plasma tPA and PAI-1, suggesting that endothelial cells could be a potential source. Patients with intravascular coagulation had significantly higher peak EVTF activity levels compared with those who did not, even after adjustment for sex and age. The peak EVTF activity value predicting intravascular coagulation was 0.51 ng/L with 63% sensitivity and 61% specificity with area under the curve = 0.63 (95% confidence interval, 0.51-0.76) and P = .046.

Conclusions: Plasma EVTF activity during HFRS is associated with intravascular coagulation.

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正表型病毒感染期间的细胞外囊泡组织因子活性与血管内凝血有关。

背景：Puumala orthohantavirus（PUUV）会引起出血热并发肾综合征（HFRS）。HFRS 患者的凝血系统处于激活状态，发生弥散性血管内凝血（DIC）和静脉血栓栓塞（VTE）的风险增加。该研究旨在确定 HFRS 患者循环中细胞外囊组织因子（EVTF）活性水平是否与 DIC 和 VTE（归类为血管内凝血）有关：方法：收集了88名HFRS患者的纵向样本。方法：我们采集了 88 名 HFRS 患者的纵向样本，将患者分为有血管内凝血（27 人）和无血管内凝血（61 人）两组。我们测量了循环EVTF活性、纤维蛋白原、活化部分凝血酶原时间、D-二聚体、组织纤溶酶原激活剂（tPA）、纤溶酶原激活剂抑制剂1（PAI-1）和血小板的水平：结果：HFRS期间血浆EVTF活性短暂升高。EVTF活性水平与血浆tPA和PAI-1显著相关，表明内皮细胞可能是潜在的来源。有血管内凝血的患者与无血管内凝血的患者相比，EVTF活性峰值水平明显更高，即使对性别和年龄进行调整后也是如此。预测血管内凝血的EVTF活性峰值为0.51纳克/升，灵敏度为63%，特异度为61%，曲线下面积=0.63（95%置信区间，0.51-0.76），P=0.046：HFRS期间血浆EVTF活性与血管内凝血有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Australian Systematic Botany 生物-进化生物学

CiteScore

3.10

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Australian Systematic Botany is an international journal devoted to the systematics, taxonomy, and related aspects of biogeography and evolution of all algae, fungi and plants, including fossils. Descriptive taxonomic papers should normally constitute a comprehensive treatment of a group. Short papers on individual species and nomenclatural papers must contain significant new information of broader interest to be considered. The prestigious L.A.S. Johnson Review Series is published. Other review articles will also be considered. All papers are peer reviewed. Australian Systematic Botany is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.