Investigation of the Effects of Direct Current Stimulation on Parkinson Disease in vitro

Bet man, M-W Rul, Bet ek, A. Lu, M. z
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Abstract

Objective: Parkinson’s disease (PD) is a progressive, neurodegenerative disease associated with the loss of dopaminergic neurons. Multiple possible mechanisms such as oxidative stress, mitochondrial dysfunction or excitotoxicity caused by glutamate are thought to mediate neuronal loss in PD. It is stated that transcranial direct current stimulation (tDCS) has positive effects on PD, but underlying mechanisms are still largely undefined. So, in this study, the effects of tDCS on PD and the relationship of these effects with glutamate and NMDA levels were investigated. Materials and Methods: To induce the PD model, 6-OHDA (200 µM) was administered to SH-SY5Y cells for 24 hours. Electrical stimulation was applied to the SH-SY5Y cells at 20 minutes and 7 hours after 24 hours. The effect of tDCS on cell viability was investigated by MTT 3-(4, 5-Dimethylthiazol-2-yl) method. Glutamate and NMDA receptor levels were measured using commercial kit. Results: It has been observed that 6-OHDA increases cell death in SH-SY5Y cells, while electrical stimulation reverses this effect. While 6-OHDA increased the glutamate level, tDCS therapy reversed this effect. No significant difference was found between the groups in NMDA levels. Conclusion: Our findings suggest that tDCS can be a functional therapy on PD by reducing glutamate toxicity.
体外直流电刺激对帕金森病影响的研究
目的:帕金森病(PD)是一种与多巴胺能神经元丧失相关的进行性神经退行性疾病。多种可能的机制,如氧化应激、线粒体功能障碍或谷氨酸引起的兴奋性毒性,被认为介导帕金森病的神经元损失。经颅直流电刺激(tDCS)对PD有积极作用,但其机制仍未明确。因此,本研究探讨了tDCS对PD的影响及其与谷氨酸和NMDA水平的关系。材料与方法:6-OHDA(200µM)作用于SH-SY5Y细胞24小时,诱导PD模型。分别于20分钟和24小时后7小时对SH-SY5Y细胞进行电刺激。采用MTT 3-(4,5 -二甲基噻唑-2-基)法研究了tDCS对细胞活力的影响。谷氨酸和NMDA受体水平采用商用试剂盒检测。结果:6-OHDA增加了SH-SY5Y细胞的死亡,而电刺激逆转了这一作用。虽然6-OHDA增加了谷氨酸水平,但tDCS治疗逆转了这一作用。各组间NMDA水平无显著性差异。结论:tDCS可通过降低谷氨酸毒性而成为PD的一种功能性治疗方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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