Ibraheem M. Attafi, Magbool E. Oraiby, Mohsen M. Fageeh, Ghassam A. Shaikhain, Mohammed Y. Albeishy, Ibrahim A. Khardali, Mohammed A. Hakami
{"title":"Post-Mortem Analysis of Diazinon and its major Metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine, in a Case of Fatal Diazinon Ingestion","authors":"Ibraheem M. Attafi, Magbool E. Oraiby, Mohsen M. Fageeh, Ghassam A. Shaikhain, Mohammed Y. Albeishy, Ibrahim A. Khardali, Mohammed A. Hakami","doi":"10.26735/16586794.2018.033","DOIUrl":null,"url":null,"abstract":"This case report describes a detection and quantitation method for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP), in postmortem blood and tissue samples from a fatal case of diazinon ingestion. Diazinon and IMHP were extracted from postmortem samples with a liquid/liquid method and analyzed by gas chromatography mass spectrometry (GC/MS). By comparing to diazinon standard and matching the retention time, diazinon was detected in two visceral organs, the stomach (0.89 μg/g) and the small intestine (8.80 μg/g). The highest level of diazinon was detected in the small intestine (8.80 μg/g), whereas the highest amount of IMHP was noted in the kidney (0.84 Area %) and bladder (0.75 Area %). In conclusion, determination of IMHP in postmortem samples could be used as an indicator for diazinon exposure, especially in the case of delayed death; whereas, the small intestine could be the best source of sample in diazinon assessment in cases of fatal diazinon ingestion.","PeriodicalId":31692,"journal":{"name":"Arab Journal of Forensic Sciences Forensic Medicine","volume":"380 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2018-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Arab Journal of Forensic Sciences Forensic Medicine","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26735/16586794.2018.033","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Social Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
This case report describes a detection and quantitation method for diazinon and its major metabolite, 2-isopropyl-4-methyl-6-hydroxypyrimidine (IMHP), in postmortem blood and tissue samples from a fatal case of diazinon ingestion. Diazinon and IMHP were extracted from postmortem samples with a liquid/liquid method and analyzed by gas chromatography mass spectrometry (GC/MS). By comparing to diazinon standard and matching the retention time, diazinon was detected in two visceral organs, the stomach (0.89 μg/g) and the small intestine (8.80 μg/g). The highest level of diazinon was detected in the small intestine (8.80 μg/g), whereas the highest amount of IMHP was noted in the kidney (0.84 Area %) and bladder (0.75 Area %). In conclusion, determination of IMHP in postmortem samples could be used as an indicator for diazinon exposure, especially in the case of delayed death; whereas, the small intestine could be the best source of sample in diazinon assessment in cases of fatal diazinon ingestion.