Strategies for minimal residual disease detection: current perspectives

IF 3.9 Q2 ONCOLOGY
G. Andreani, D. Cilloni
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引用次数: 4

Abstract

Abstract Currently, the post-remission treatment in acute leukemia is based on the genetic profile of leukemic cells at diagnosis (ie, FLT3 ITD positivity) and on the level of measurable residual disease (MRD) after induction and consolidation chemotherapy. Two methods are currently preferred for MRD evaluation in many centers: multiparameter flow cytometry and real-time quantitative PCR. Additional methods such as next-generation sequencing and digital PCR are under investigation, in an attempt to increase the sensitivity and thus allowing the detection of small clones. Many studies suggest that MRD positivity after chemotherapy is associated with negative prognosis, and the reappearance of MRD during follow-up allows impending relapse to be identified and consequently enables early intervention. Finally, MRD positivity before hematopoietic stem cell transplantation is predictive of the outcome. Although the significance of MRD in acute leukemia has been widely explored, the assessment of molecular MRD is not yet a routine practice. In this review, we describe the significance of MRD in different settings and the main markers and methods used for MRD detection.
最小残留疾病检测策略:当前观点
目前,急性白血病的缓解后治疗是基于诊断时白血病细胞的遗传谱(即FLT3 ITD阳性)和诱导和巩固化疗后可测量的残留病(MRD)水平。目前在许多中心首选的MRD评估方法有两种:多参数流式细胞术和实时定量PCR。其他方法,如下一代测序和数字PCR正在研究中,试图提高灵敏度,从而允许检测小克隆。许多研究表明,化疗后MRD阳性与不良预后相关,随访期间MRD的复发可以识别即将复发,从而使早期干预成为可能。最后,造血干细胞移植前MRD阳性可预测结果。虽然MRD在急性白血病中的意义已被广泛探讨,但分子MRD的评估尚未成为常规做法。在这篇综述中,我们描述了MRD在不同情况下的意义以及MRD检测的主要标记物和方法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
自引率
7.10%
发文量
16
审稿时长
16 weeks
期刊介绍: Blood and Lymphatic Cancer: Targets and Therapy is an international, peer reviewed, open access journal focusing on blood and lymphatic cancer research, identification of therapeutic targets, and the optimal use of preventative and integrated treatment interventions to achieve improved outcomes, enhanced survival, and quality of life for the cancer patient. Specific topics covered in the journal include: Epidemiology, detection and screening Cellular research and biomarkers Identification of biotargets and agents with novel mechanisms of action Optimal clinical use of existing anticancer agents, including combination therapies Radiation, surgery, bone marrow transplantation Palliative care Patient adherence, quality of life, satisfaction Health economic evaluations.
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