Synthesis and study of a disinfectant based on diethanolamine

Abstract

The interaction of diethanolamine with formaldehyde at a ratio of initial reagents of 2?:?3, respectively, obtained a preparation that is an equimolar mixture of 3-(?-hydroxyethyl)-1,3-oxazolidine and its methylol derivative – 3-(?-hydroxyoxymethyl-ethyl)-1,3-oxazolidine. The paper presents the results of a study of the antimicrobial activity of the preparation against microorganisms – S. aureus, E. coli, as the most resistant species of bacteria that cause nosocomial infection. The tests were carried out by three methods: the test object method, as well as suspension and adsorption methods. The minimum effective concentration and exposure time of the preparation, which ensure the complete destruction of these microorganisms, have been established. The effect of organic substances and neutralizers on the antimicrobial activity of the preparation was studied. «Sulfonol» (a mixture of stereoisomers of the sodium salt of alkylbenzenesulfonic acid) at a concentration of 0,2?% and ten percent milk were used as neutralizers, and 10?% horse serum was used as protein. The concentration of the preparation and the exposure time required for the complete disinfection of dishes, glass and metlakh tiles infected with S. aureus and E. coli were determined. Disinfection of surfaces is achieved by treatment with a 3?% solution of the preparation during an exposure of 60 minutes, and dishes – with 3–5?% solutions for 45 minutes. It has been established by the thermoanalytical method of analysis that the preparation is stable at normal temperature and does not change its physicochemical properties during storage for 18 months or more. The preparation is recommended as a disinfectant for medical purposes. Keywords: 1,3-oxazolidine derivatives, 3-(?-hydroxyethyl)-1,3-oxazolidine, 3-(?-hydroxyoxymethylethyl)-1,3-oxazolidine, antimicrobial activity, bactericidal activity, nosocomial infection, bacteria, S. aureus, E. coli, disinfection, disinfectant.