Chan Hum Park , Dong Young Rhyu , Bhesh Raj Sharma , Takako Yokozawa
{"title":"Inhibition of preadipocyte differentiation and lipid accumulation by 7-O-galloyl-d-sedoheptulose treatment in 3T3-L1 adipocytes","authors":"Chan Hum Park , Dong Young Rhyu , Bhesh Raj Sharma , Takako Yokozawa","doi":"10.1016/j.bionut.2013.03.012","DOIUrl":null,"url":null,"abstract":"<div><p>In this study, we investigated the effect of 7-<em>O</em>-galloyl-<span>d</span><span><span><span>-sedoheptulose (GS) on adipocyte differentiation in 3T3-L1 cells. GS significantly suppressed 3T3-L1 differentiation. The activation of adenosine monophosphate-activated </span>protein kinase<span> (AMPK) was demonstrated by measuring phosphorylation. In addition, GS down-regulated the adipogenesis-related protein expression of the sterol regulatory element-binding protein 1c. GS significantly reduced the protein expression of the peroxisome proliferator-activated receptor γ and of the CCAAT/enhancer-binding protein α, two key transcription factors in </span></span>adipogenesis. These findings indicate that GS exerts anti-adipogenic activity </span><em>via</em> AMPK activation and may act to prevent obesity.</p></div>","PeriodicalId":100182,"journal":{"name":"Biomedicine & Preventive Nutrition","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2013-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bionut.2013.03.012","citationCount":"11","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomedicine & Preventive Nutrition","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S221052391300024X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 11
Abstract
In this study, we investigated the effect of 7-O-galloyl-d-sedoheptulose (GS) on adipocyte differentiation in 3T3-L1 cells. GS significantly suppressed 3T3-L1 differentiation. The activation of adenosine monophosphate-activated protein kinase (AMPK) was demonstrated by measuring phosphorylation. In addition, GS down-regulated the adipogenesis-related protein expression of the sterol regulatory element-binding protein 1c. GS significantly reduced the protein expression of the peroxisome proliferator-activated receptor γ and of the CCAAT/enhancer-binding protein α, two key transcription factors in adipogenesis. These findings indicate that GS exerts anti-adipogenic activity via AMPK activation and may act to prevent obesity.
在本研究中,我们研究了7- o -没食子酰-d-sedoheptulose (GS)对3T3-L1细胞脂肪细胞分化的影响。GS显著抑制3T3-L1分化。腺苷单磷酸活化蛋白激酶(AMPK)的活化是通过测量磷酸化证实的。此外,GS还下调了脂肪形成相关蛋白中固醇调节元件结合蛋白1c的表达。GS显著降低了过氧化物酶体增殖物激活受体γ和CCAAT/增强子结合蛋白α的蛋白表达,这是脂肪形成的两个关键转录因子。这些发现表明,GS通过激活AMPK发挥抗脂肪生成活性,并可能起到预防肥胖的作用。