Antioxidant Potential and Total Phenolic Compounds of Extracts and Fractions of Pistasia atlantica

Pharmaceutical and Clinical Research Pub Date : 2017-04-25 DOI:10.25258/IJPCR.V9I04.8536

S. Alidadi, M. Moradi, M. Asadi-Samani, Z. Lorigooini

{"title":"Antioxidant Potential and Total Phenolic Compounds of Extracts and Fractions of Pistasia atlantica","authors":"S. Alidadi, M. Moradi, M. Asadi-Samani, Z. Lorigooini","doi":"10.25258/IJPCR.V9I04.8536","DOIUrl":null,"url":null,"abstract":"Different parts of Pistasia atlantica have been used in traditional medicine for various purposes in Iran. The aim of this\nstudy was to measurement and compare antioxidant activity and polyphenolic compounds of crude ethyl alcohol extract\nand four fractions of P. atlantica leaf. Crude ethyl alcohol extract of P. atlantica leaf was prepared using maceration\nmethod and subjected to fractionation with different polarity. The antioxidant potential of all these fractions was evaluated\nby the 2,2 diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. The total phenolic, flavonoid, and\nflavonol components were measured with Folin-Ciocaltiue and Chlorid Aluminum methods. According to the radical\nscavenging capacity, the ethyl acetate fraction exhibited the highest antioxidant activity with IC50 value 1.54±0.12 µg/ml,\nfollowed by the chloroform fraction with higher percent inhibition of the DPPH with 3.4±0.11 µg/ml. The results are\nrepresented relative to a reference standard, butylated hydroxytoluene (BHT), with IC50 value of 33.5±3.67μg/ml. Among\nthese fractions, the ethyl acetate fraction and chloroform fraction had the highest amount of total phenolic compounds with\nvalue of 532.73 and 355.14 mg GAE/g, respectively. The results of this study showed that some fractions of P. atlantica\nleaf extract could be used as easily accessible source of natural antioxidants","PeriodicalId":19889,"journal":{"name":"Pharmaceutical and Clinical Research","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pharmaceutical and Clinical Research","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.25258/IJPCR.V9I04.8536","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 9

Abstract

Different parts of Pistasia atlantica have been used in traditional medicine for various purposes in Iran. The aim of this study was to measurement and compare antioxidant activity and polyphenolic compounds of crude ethyl alcohol extract and four fractions of P. atlantica leaf. Crude ethyl alcohol extract of P. atlantica leaf was prepared using maceration method and subjected to fractionation with different polarity. The antioxidant potential of all these fractions was evaluated by the 2,2 diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging activity method. The total phenolic, flavonoid, and flavonol components were measured with Folin-Ciocaltiue and Chlorid Aluminum methods. According to the radical scavenging capacity, the ethyl acetate fraction exhibited the highest antioxidant activity with IC50 value 1.54±0.12 µg/ml, followed by the chloroform fraction with higher percent inhibition of the DPPH with 3.4±0.11 µg/ml. The results are represented relative to a reference standard, butylated hydroxytoluene (BHT), with IC50 value of 33.5±3.67μg/ml. Among these fractions, the ethyl acetate fraction and chloroform fraction had the highest amount of total phenolic compounds with value of 532.73 and 355.14 mg GAE/g, respectively. The results of this study showed that some fractions of P. atlantica leaf extract could be used as easily accessible source of natural antioxidants

查看原文本刊更多论文

大西洋pistasianatlantica提取物和馏分的抗氧化能力和总酚类化合物研究

在伊朗，Pistasia atlantica的不同部分在传统医学中用于各种目的。本研究的目的是测定和比较大西洋叶的粗乙醇提取物和四个部分的抗氧化活性和多酚类化合物。采用浸渍法制备了大西洋叶粗乙醇提取物，并进行了不同极性的分馏。采用2,2二苯基-1-苦味酰肼(DPPH)自由基清除活性法评价各组分的抗氧化能力。用福林- ciocalue法和氯铝法测定了总酚、类黄酮和黄酮醇成分。根据自由基清除能力，乙酸乙酯部位抗氧化活性最强，IC50值为1.54±0.12µg/ml，氯仿部位次之，IC50值为3.4±0.11µg/ml。结果与对照品丁基羟基甲苯(BHT)的IC50值为33.5±3.67μg/ml。其中乙酸乙酯部位和氯仿部位总酚类化合物含量最高，分别为532.73和355.14 mg GAE/g。本研究结果表明，大西洋叶提取物的部分组分可作为易获取的天然抗氧化剂来源

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Pharmaceutical and Clinical Research

自引率

0.00%

发文量

4693