Molecular Method to Diagnosis of some Strongylide Nematode of goats in Nyala Area South Darfur State- Sudan

Abstract

A total of 100 positive faecal samples were examined using floatation technique to detect strongylide eggs, were confirmed by PCR technique, faecal samples were storage by frozen in -20 ° C and DNA extraction by using phenol/chloroform/isoamylalcohol protocol. PCR leading to amplify the region of the second internal transcribed spacer (ITS-2) of nuclear ribosomal DNA (r DNA) of strongylide species, amplification by three different primers in separate reactions, in each reaction used species specific primer forward and a universal reverse primer to srongylide species NC2. Haemonchus contortus, Trichostrongylus spp and Oesophagostomum columbianum prevalence rates were recorded as 47(47%), 4(4%) and (0%) respectively, Most commonly, small ruminants are affected by multiple strongylide nematodes, mixed infection between Haemonchus contortus and Trichostrongylus spp was recorded 3(3%) Keyword: Strongylide, PCR, ITS-2, NC2, goats, Sudan.