Evaluation of the Role of Vitamin C and Melatonin on the Genetic Disorder of Human Blood Lymphocytes in the Presence of Vincristine and Permethrin

M. Shokrzadeh, P. Mortazavi, E. Karimi, Behnam NasirOghli, Shaghayegh Shokrzadeh, F. Motafeghi
{"title":"Evaluation of the Role of Vitamin C and Melatonin on the Genetic Disorder of Human Blood Lymphocytes in the Presence of Vincristine and Permethrin","authors":"M. Shokrzadeh, P. Mortazavi, E. Karimi, Behnam NasirOghli, Shaghayegh Shokrzadeh, F. Motafeghi","doi":"10.18502/tbsrj.v3i3.6986","DOIUrl":null,"url":null,"abstract":"Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on the body's normal cells. Due to the widespread use of these drugs, preventing their toxicity is essential; therefore, in this study, the protective effects of vitamin C and melatonin on the genetic toxicity induced by vincristine and permethrin in peripheral blood lymphocytes were investigated. \nMaterial and Methods: The protective effects of vitamin C and melatonin (doses of 50, 100 and 200 µm) on the toxicity of vincristine and permethrin induced by micronucleus test on peripheral blood lymphocytes were evaluated ,and in statistical tests P <0.05 as the significant level was considered. \nResults: According to the results vincristine and permethrin caused genetic disorder by 28.80 ± 1.92 and 34±1.58 micronucleus respectively (p<0.0001). However, by exposing vitamin c and melatonin with permethrin at concentrations of 100 μM and 200 μM, the number of micronuclei was significantly decreased by 24.80 ±2.91, 18.00±1.58 (Vit C) and 22.20±3.34, 15.40±1.14 (melatonin) respectively. In contrast, exposure of these two substances together with vincristine in similar concentrations reduced the micronucleus by 16.60± 2.07 ,10.80± 0.83 (Vit C) and 13.00± 1.58, 6.40± 1.14 (melatonin) respectively. \nConclusion: As the results of this study showed, permethrin and vincristine both caused genetic toxicity. Melatonin can protect against DNA damage by purifying reactive oxygen species or stimulating the DNA repair system. Vitamin C plays an essential protective role in many toxic reactions of the body. Both antioxidants have been shown to reduce the genetic toxicity of permethrin and vincristine.","PeriodicalId":22117,"journal":{"name":"Tabari Biomedical Student Research Journal","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tabari Biomedical Student Research Journal","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/tbsrj.v3i3.6986","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

Introduction: Various studies have shown that vincristine and permethrin have genetic toxicity on the body's normal cells. Due to the widespread use of these drugs, preventing their toxicity is essential; therefore, in this study, the protective effects of vitamin C and melatonin on the genetic toxicity induced by vincristine and permethrin in peripheral blood lymphocytes were investigated. Material and Methods: The protective effects of vitamin C and melatonin (doses of 50, 100 and 200 µm) on the toxicity of vincristine and permethrin induced by micronucleus test on peripheral blood lymphocytes were evaluated ,and in statistical tests P <0.05 as the significant level was considered. Results: According to the results vincristine and permethrin caused genetic disorder by 28.80 ± 1.92 and 34±1.58 micronucleus respectively (p<0.0001). However, by exposing vitamin c and melatonin with permethrin at concentrations of 100 μM and 200 μM, the number of micronuclei was significantly decreased by 24.80 ±2.91, 18.00±1.58 (Vit C) and 22.20±3.34, 15.40±1.14 (melatonin) respectively. In contrast, exposure of these two substances together with vincristine in similar concentrations reduced the micronucleus by 16.60± 2.07 ,10.80± 0.83 (Vit C) and 13.00± 1.58, 6.40± 1.14 (melatonin) respectively. Conclusion: As the results of this study showed, permethrin and vincristine both caused genetic toxicity. Melatonin can protect against DNA damage by purifying reactive oxygen species or stimulating the DNA repair system. Vitamin C plays an essential protective role in many toxic reactions of the body. Both antioxidants have been shown to reduce the genetic toxicity of permethrin and vincristine.
维C和褪黑素对长春新碱和氯菊酯存在下人血液淋巴细胞遗传性疾病的影响
各种研究表明长春新碱和氯菊酯对人体正常细胞具有遗传毒性。由于这些药物的广泛使用,预防其毒性至关重要;因此,本研究探讨维生素C和褪黑素对长春新碱和氯菊酯诱导的外周血淋巴细胞遗传毒性的保护作用。材料与方法:评价维生素C和褪黑素(剂量分别为50、100和200µm)对长春新碱和氯菊酯微核试验诱导的外周血淋巴细胞毒性的保护作用,在统计学检验中以P <0.05为显著水平。结果:长春新碱和氯菊酯分别造成28.80±1.92和34±1.58微核遗传障碍(p<0.0001)。氯菊酯浓度为100 μM和200 μM时,维生素c和褪黑激素的微核数量分别减少24.80±2.91、18.00±1.58 (Vit c)和22.20±3.34、15.40±1.14(褪黑激素)。相比之下,这两种物质与相同浓度的长春新碱一起暴露时,微核分别减少16.60±2.07,10.80±0.83 (Vit C)和13.00±1.58,6.40±1.14(褪黑素)。结论:氯菊酯和长春新碱均具有遗传毒性。褪黑素可以通过净化活性氧或刺激DNA修复系统来防止DNA损伤。维生素C在人体的许多毒性反应中起着至关重要的保护作用。这两种抗氧化剂都被证明可以降低氯菊酯和长春新碱的遗传毒性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信