Development and Validation of Double Divisor Ratio Spectra Derivative Method for Simultaneous Estimation of Telmisartan, Chlorthalidone and Cilnidipine in Tablet Dosage Form

Abstract

: Aim: Development and Validation of Double Divisor Ratio Spectra Derivative Method for Simultaneous Estimation of Telmisartan, Chlorthalidone and Cilnidipine in Tablet Dosage Form. Method: A new method was developed for the simultaneous quantification of ternary mixtures by double divisor spectra method and validated as per International Conference on Harmonization [(ICH) Q2 (R1)] guideline. In double divisor spectra method absorption spectrum of ternary mixture divided by two of the three compounds in the mixture. Methanol used as solvent for this method. Calibration graphs were established in the range 10-50 μg/mL for Telmisartan, 7.5-17.5 μg/mL for Chlorthalidone and 5-25 μg/mL for Cilnidipine. Results: all the drugs exhibited good linearity over the reported concentration range with acceptable correlation coefficient. The method was validated according to ICH guideline parameters such as accuracy, repeatability, reproducibility showing acceptable percent relative standard deviation of less than 2. Conclusion: The proposed method demonstrated that the method is accurate, simple, precise, compounds not interference with each other and no any prior separation of compounds. © 2020 iGlobal Research and Publishing Foundation.