Antibiotic resistance profile of Escherichia coli isolated from apparently healthy domestic livestock in South-East Nigeria

Chijioke A. Nsofor, C. U. Iroegbu
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引用次数: 15

Abstract

This study was conducted to determine the antibiotic resistance profile of Escherichia coli isolated from apparently healthy domestic livestock viz: cattle, goats, poultry and swine from three states in South East of Nigeria. E. coli was isolated using Eosin Methylene Blue agar (EMB) and identified by conventional microbiological technique. The isolates were tested against 14 antibiotics using the disc diffusion method. A total of 42 different antibiotics resistance profiles were observed, with each isolate showing resistance to at least four or more drugs tested. Generally, the 80 E. coli isolates showed resistance rates of 85% to Ampicillin; 90% to Cotrimaoxazole, 90% to Cephalothin; 77.5% to Streptomycin, 62.5% to Nitrofurantoin; 68.8% to Tetracycline; 55% to Chloramphenicol; 56.3% to Amoxicillin clavulanic acid; 58.8% to Cefpirome; 47.5% to Cefpodoxime; 43.8% to Cefotaxine; 22.5% to Ceftriaxone; 18.8% to Cefoxitin; 16.3% to Nalidixic acid and 12.5% to Gentamycin. Statistical analysis showed that average number of resistance phenotypes per isolate was significantly higher for goat and poultry compared with cattle and swine (P<0.0001). A significant public health concern observed in this study is that multidrug-resistant commensal E. coli strains may constitute a potential reservoir of resistance genes that could be transferred to pathogenic bacteria.   Key words: Antibiotic resistance, Escherichia coli, Nigeria.
从尼日利亚东南部明显健康的家畜中分离出的大肠杆菌的抗生素耐药性谱
进行这项研究是为了确定从尼日利亚东南部三个州明显健康的家畜(即牛、山羊、家禽和猪)中分离的大肠杆菌的抗生素耐药性谱。采用伊红亚甲基蓝琼脂(EMB)分离大肠杆菌,采用常规微生物学技术进行鉴定。采用圆盘扩散法对分离菌株进行了14种抗菌药物的检测。总共观察到42种不同的抗生素耐药谱,每种分离株对至少四种或更多种药物具有耐药性。总的来说,80株大肠杆菌对氨苄青霉素的耐药率为85%;复方新恶唑90%头孢菌素90%链霉素占77.5%,呋喃妥因占62.5%;68.8%选择四环素;氯霉素55%;阿莫西林克拉维酸占56.3%;头孢匹罗占58.8%;头孢多肟占47.5%;头孢他辛占43.8%;头孢曲松22.5%;头孢西丁占18.8%;那利地酸占16.3%庆大霉素占12.5%统计分析表明,山羊和家禽的平均抗性表型数显著高于牛和猪(P<0.0001)。在这项研究中观察到的一个重要的公共卫生问题是,多重耐药共生大肠杆菌菌株可能构成一个潜在的耐药基因库,可以转移到致病菌。关键词:抗生素耐药性;大肠杆菌;尼日利亚;
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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