Population structure, genetic diversity and bakanae disease resistance among rice varieties

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-03-30 DOI:10.1017/s1479262123000199

S. Raghu, M. Baite, M. Yadav, S. R. Prabhukarthikeyan, U. Keerthana, C. Anil Kumar, B. Jeevan, S. Lenka, H. Subudhi, P. Rath

{"title":"Population structure, genetic diversity and bakanae disease resistance among rice varieties","authors":"S. Raghu, M. Baite, M. Yadav, S. R. Prabhukarthikeyan, U. Keerthana, C. Anil Kumar, B. Jeevan, S. Lenka, H. Subudhi, P. Rath","doi":"10.1017/s1479262123000199","DOIUrl":null,"url":null,"abstract":"\n Availability of resistance sources among cultivated varieties helps in easy utilization as donor owing to no deleterious linkage drag. In the present investigation, 121 rice varieties were screened for their resistance against a virulent isolate of Fusarium fujikuroi (Ff-10) and genotyped using reported microsatellite markers. Among 121 varieties, only eight varieties, namely Luna Sankhi, Improved Tapaswini, Sarasa, Sadabahar, CR-311, Kshira, Wifa-10 and Binadhan-8, were found to be highly resistant (HR), seven varieties were resistant (R), 31 were moderately resistant (MR), 10 were moderately susceptible (MS), 11 were susceptible (S) and the rest 54 were highly susceptible (HS). The allele diversity of molecular markers classified the population into three clusters. The highly resistant varieties were grouped in major clusters II and III, whereas the remaining genotypes were distributed in all three clusters. Analysis of molecular variance (AMOVA) resulted in 95% of the maximum diversity within the test population and 5% diversity between populations. Population structure analysis grouped the genotypes into two sub-populations based on relatedness, where most of the resistant genotypes were grouped into one sub-population and other genotypes were distributed among sub-populations. Re-examination of reported markers' trait associations with bakanae resistance in the experimental population identified marker RM-3698 as associated with resistance accounting 8.4% explained phenotypic variation. This study shows that simple sequence repeat markers can be used to assess allelic diversity and population structure of bakanae resistance in rice varieties. The highly resistant genotypes, along with resistance markers, could be used as donors in marker-assisted bakanae improvement breeding programmes.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1017/s1479262123000199","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Availability of resistance sources among cultivated varieties helps in easy utilization as donor owing to no deleterious linkage drag. In the present investigation, 121 rice varieties were screened for their resistance against a virulent isolate of Fusarium fujikuroi (Ff-10) and genotyped using reported microsatellite markers. Among 121 varieties, only eight varieties, namely Luna Sankhi, Improved Tapaswini, Sarasa, Sadabahar, CR-311, Kshira, Wifa-10 and Binadhan-8, were found to be highly resistant (HR), seven varieties were resistant (R), 31 were moderately resistant (MR), 10 were moderately susceptible (MS), 11 were susceptible (S) and the rest 54 were highly susceptible (HS). The allele diversity of molecular markers classified the population into three clusters. The highly resistant varieties were grouped in major clusters II and III, whereas the remaining genotypes were distributed in all three clusters. Analysis of molecular variance (AMOVA) resulted in 95% of the maximum diversity within the test population and 5% diversity between populations. Population structure analysis grouped the genotypes into two sub-populations based on relatedness, where most of the resistant genotypes were grouped into one sub-population and other genotypes were distributed among sub-populations. Re-examination of reported markers' trait associations with bakanae resistance in the experimental population identified marker RM-3698 as associated with resistance accounting 8.4% explained phenotypic variation. This study shows that simple sequence repeat markers can be used to assess allelic diversity and population structure of bakanae resistance in rice varieties. The highly resistant genotypes, along with resistance markers, could be used as donors in marker-assisted bakanae improvement breeding programmes.

查看原文本刊更多论文

水稻品种间群体结构、遗传多样性与白僵病抗性

栽培品种间抗性源的可用性，由于没有有害的连锁阻力，有助于作为供体的利用。在本研究中，对121个水稻品种进行了抗性筛选，并利用报道的微卫星标记进行了基因分型。121个品种中，只有Luna Sankhi、改良Tapaswini、Sarasa、Sadabahar、CR-311、Kshira、Wifa-10和Binadhan-8 8个品种为高抗性(HR)， 7个品种为抗性(R)， 31个品种为中抗性(MR)， 10个品种为中敏感(MS)， 11个品种为敏感(S)，其余54个品种为高敏感(HS)。分子标记的等位基因多样性将种群划分为3个聚类。高抗性品种主要分布在第2和第3大群中，其余基因型分布在所有3个群中。分子方差分析(AMOVA)结果表明，受试群体内的最大多样性为95%，群体间的最大多样性为5%。群体结构分析根据亲缘关系将耐药基因型分为两个亚群体，其中大部分基因型集中在一个亚群体中，其他基因型分布在不同的亚群体中。对实验群体中已报道的与bakanae抗性相关的标记性状进行重新检查，发现标记RM-3698与抗性相关，占表型变异解释的8.4%。本研究表明，利用简单序列重复标记可以评价水稻品种对bakanae抗性的等位基因多样性和群体结构。高抗性基因型以及抗性标记可以作为标记辅助的bakanae改良育种计划的供体。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.