The effect of aerobic and anaerobic exercise on bax and bcl-2 myocardium apoptotic markers after reperfusion ischemia

Abstract

The present research aims to examine the effect of aerobic and anaerobic exercise on Bax and Bcl-2 markers after the induced reperfusion ischemia by isoprenaline. In this study, there were used 26 male Wistar rats weighing approximately 200-250 g with two to three months old. The rats in pilot group were divided into two subgroups of reperfusion ischemia (n= 7) and healthy (n= 7) for study of heart-failure byisoprenaline. The rats in research group were divided into three subgroups ofaerobic (n= 4), anaerobic (n= 4) and control (n= 4) for study of gene expression. In rats of reperfusion ischemia group were confirmed heart-failure after injection isoprenaline by Trichromation staining technique. However, the rats in research group were familiar running on treadmill after one week, then they were exposed for one month training with frequency of three times weekly. After one month, all rats were rest for two consecutive days. Then within 24 hours, there were injected isoprenaline with dose of 150 and 125 mg per kg of their body weight. Data was analyzed using independent T, one way ANOVA and2 ct formula. The research results showed that aerobic and anaerobic exercise can reduce and increase Bax and Bcl-2 gene expression respectively, but these changes were not significant. However, it seems that one month training can’t decrease amount of myocardial apoptosis. Therefore, study of effect increase of period physical activity is essential for decrease amount of heart-failure.