Biosystematics, fingerprinting and DNA barcoding study of the genus Lallemantia based on SCoT and REMAP markers

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-03-08 DOI:10.36253/caryologia-1163

F. Koohdar, N. Aram, M. Sheidai

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引用次数: 1

Abstract

Lallemantia is a medicinally important plant in the world. Due to interspecific hybridization and horizontal gene transfer, spices relationship and delimitation on the genus Lallemantia is difficult based on different molecular markers. Therefore, selecting the appropriate marker can be important. Fingerprinting techniques continue to be used for genomic profiling for the characterization of germplasm and the establishment of the identity of varieties/hybrids/parental sources of aromatic and medicinal plants. For this, we need to produce detailed information on genetic diversity available in Lallemantia as well as investigate spices relationship and delimitation. Therefore, the present study was performed on Lallemantia species in Iran. We used the start codon targeted and retrotransposon-microsatellite amplified polymorphism molecular marker for our genetic investigation with the following aims: 1- To reveal the species delimitation and species relationship in Lallemantia, and 2- To investigate discriminating power of the start codon targeted and retrotransposon-microsatellite amplified polymorphism markers by Gst and NM analysis. The results obtained revealed that the start codon targeted marker is the best to show the relationships between species while the retrotransposon-microsatellite amplified polymorphism marker is the best for species delimitation. we found the loci with the high value of Gst (1.00) in start codon targeted and retrotransposon-microsatellite amplified polymorphism markers that can be used in barcoding and fingerprinting of L. royleana.

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基于SCoT和REMAP标记的拉曼草属生物系统、指纹图谱和DNA条形码研究

芒草是世界上一种重要的药用植物。由于种间杂交和基因的水平转移，在不同分子标记的基础上，苦苣苔属香料的亲缘关系和界别存在困难。因此，选择合适的标记非常重要。指纹图谱技术继续被用于种质资源的基因组分析，以及芳香和药用植物品种/杂交/亲本来源的鉴定。为此，我们需要对拉曼提亚现有的遗传多样性提供详细的信息，并研究香料的关系和划分。因此，本研究以伊朗的Lallemantia种为研究对象。本研究利用起始密码子靶向和反转录转座子微卫星扩增多态性分子标记进行遗传研究，目的是:1-揭示Lallemantia属的物种划分和物种关系;2-通过Gst和NM分析，研究起始密码子靶向和反转录转座子微卫星扩增多态性分子标记的鉴别能力。结果表明，起始密码子靶向标记最能显示物种间的关系，而反转录转座子微卫星扩增多态性标记最能区分物种。我们在开始密码子靶向标记和反转录转座子微卫星扩增多态性标记中发现了Gst值高(1.00)的位点，可用于罗氏菌的条形码和指纹识别。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.