Molecular cloning and sequence analysis of 1Dx-type HMW-GS genes from different wheat varieties

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-10-28 DOI:10.1017/s1479262122000211

Li-Juan Yang, Xiang-Fen Zhang, Yan-Qi Dong, Yue-Ting Zheng, Jin-Xia Zhang, Yi He, Xiu-Fang Tan, Zhi-Kai Jiang, Hua-Ping Ma

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引用次数: 0

Abstract

High-molecular-weight glutenin subunits (HMW-GS) contribute to dough elasticity and bread baking quality in wheat. In this study, wheat varieties were classified based on their HMW-GS composition into three groups: 1Dx5 (5 + 10, Gaoyou 8901, Xinmai 28, Xinmai 19, Xinmai 26 and Jinbaoyin), 1Dx2 (2 + 12, Zhoumai 24, Xinmai 9 and Yumai) and 1Dx4 (4 + 12, Aikang 58). Sequence analysis showed that 1Dx-GY8901, 1Dx-XM28, 1Dx-XM19 and 1Dx-XM26 were similar to the 1Dx5 gene and clustered on the same branch, while 1Dx-AK58, 1Dx-ZM24, 1Dx-JBY, 1Dx-YM, 1Dx-XM9 and 1Dx-JBY were more similar to the 1Dx2 gene and clustered on the same branch with 1Dx.2.2. There was a mutation of Ser to Cys at position S2, for an extra Cys in the repeat regions of 1Dx-XM19, 1Dx-XM26, 1Dx-XM28 and 1Dx-GY8901. The wheat HMW-GS genes exhibited similar percentages of α-helix, extended strand, β-turn and random coil structure, with ranges of 13.33–13.59, 4.77–5.78, 7.08–9.18 and 72.3–73.94%, respectively. Sequence conservation and the composition of HMW-GS subunits were also analysed for a series of strong gluten wheat varieties, Xinmai 9 (1, 7 + 8, 2 + 12), Xinmai 19 (1, 7 + 9, 5 + 10), Xinmai 26 (1, 7 + 8, 5 + 10) and Xinmai 28 (1, 7 + 9, 5 + 10). The results of this work should facilitate future breeding efforts and provide the theoretical basis for wheat quality improvement.

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不同小麦品种1dx型HMW-GS基因的克隆与序列分析

高分子量谷蛋白亚基(HMW-GS)对小麦面团弹性和面包烘焙品质有重要影响。本研究根据小麦品种的HMW-GS组成将小麦品种分为3组:1Dx5(5 + 10、高邮8901、新麦28、新麦19、新麦26和金宝银)、1Dx2(2 + 12、周麦24、新麦9和玉麦)和1Dx4(4 + 12、爱康58)。序列分析表明，1Dx-GY8901、1Dx-XM28、1Dx-XM19和1Dx-XM26与1Dx5基因相似，聚在同一支上，而1Dx-AK58、1Dx-ZM24、1Dx-JBY、1Dx-YM、1Dx-XM9和1Dx-JBY与1Dx2基因更相似，与1Dx.2.2聚在同一支上。在1Dx-XM19、1Dx-XM26、1Dx-XM28和1Dx-GY8901的重复区，Ser在S2位置突变为Cys，增加了一个Cys。小麦HMW-GS基因α-螺旋结构、延伸链结构、β-转结构和随机螺旋结构的比例相近，分别为13.33 ~ 13.59、4.77 ~ 5.78、7.08 ~ 9.18和72.3 ~ 73.94%。对新麦9号(1,7 + 8,2 + 12)、新麦19号(1,7 + 9,5 + 10)、新麦26号(1,7 + 8,5 + 10)和新麦28号(1,7 + 9,5 + 10)的序列保守性和HMW-GS亚基组成进行了分析。研究结果可为今后的育种工作提供参考，并为小麦品质改良提供理论依据。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.