Molecular Epidemiological Investigation of Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. Infection in Free-ranged Yaks and Tibetan Pigs on the Plateau

The Pakistan Veterinary Journal Pub Date : 2022-10-01 DOI:10.29261/pakvetj/2022.060

Xiu P. Chen

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引用次数: 6

Abstract

Intestinal parasites are of great economic importance in livestock. However, scarcity of data has been found about the prevalence of four important intestinal parasites including Cryptosporidium sp., Giardia duodenalis, Enterocytozoon bieneusi and Blastocystis sp. infection in free-ranged yaks and Tibetan pigs during the winter season on the plateau. Fecal samples of yaks (n=40) and Tibetan pigs (n=60) were collected and molecular identification of these parasites was performed through nested PCR amplification. Positive PCR samples were sequenced and further phylogenetic analysis was performed. Results found that the prevalence of Cryptosporidium sp., G. duodenalis, E. bieneusi and Blastocystis sp. was 10.0, 7.5, 12.5, 7.5% in yaks, and 18.3, 0, 56.7, 50.0% in Tibetan pigs, respectively. Co-infections was found between 2.5-7.5% in yaks and 0-26.7% in Tibetan pigs, respectively. The sequenced samples were identified to be Cryptosporidium sp. and was identified as pig genotype II. Findings of this study will provide an insight to the prevention and control for these important parasites on the high plateau

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高原散养牦牛和藏猪隐孢子虫、十二指肠贾第鞭毛虫、双胞虫和囊虫感染的分子流行病学调查

肠道寄生虫对家畜具有重要的经济意义。然而，关于青藏高原冬季散养牦牛和藏猪中隐孢子虫、十二指肠贾第鞭毛虫、双氏肠胞虫和囊虫等4种重要肠道寄生虫的感染情况缺乏相关数据。收集牦牛(n=40)和藏猪(n=60)粪便标本，采用巢式PCR扩增方法对寄生虫进行分子鉴定。对阳性PCR样本进行测序，并进行进一步的系统发育分析。结果牦牛隐孢子虫、十二指肠棘球绦虫、布氏绦虫和囊虫感染率分别为10.0%、7.5%、12.5和7.5%，藏猪为18.3%、0%、56.7和50.0%。牦牛共感染2.5 ~ 7.5%，藏猪共感染0 ~ 26.7%。经测序鉴定为猪隐孢子虫，属猪基因型II型。本研究结果将为高原重要寄生虫的防治提供参考

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The Pakistan Veterinary Journal

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