Trichoderma sp. spores and Kluyveromyces marxianus cells magnetic separation: Immobilization on chitosan-coated magnetic nanoparticles

Sócrates Palácios-Ponce, Rodolfo Ramos‐González, H. Ruiz, M. Aguilar, J. Martínez-Hernández, E. P. Segura-Ceniceros, C. N. Aguilar, G. Michelena, A. Ilyina
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引用次数: 6

Abstract

ABSTRACT In the present study, the interactions between chitosan-coated magnetic nanoparticles (C-MNP) and Trichoderma sp. spores as well as Kluyveromyces marxianus cells were studied. By Plackett–Burman design, it was demonstrated that factors which directly influenced on yeast cell immobilization and magnetic separation were inoculum and C-MNP quantity, stirring speed, interaction time, and volume of medium, while in the case of fungal spores, the temperature also was disclosed as an influencing factor. Langmuir and Freundlich models were applied for the mathematical analysis of adsorption isotherms at 30°C. For Trichoderma sp. spore adsorption isotherm, the highest correlation coefficient was observed for lineal function of Langmuir model with a maximum adsorption capacity at 5.00E + 09 spores (C-MNP g−1). Adsorption isotherm of K. marxianus cells was better adjusted to Freundlich model with a constant (Kf) estimated as 2.05E + 08 cells (C-MNP g−1). Both systems may have a novel application in fermentation processes assisted with magnetic separation of biomass.
木霉孢子和马氏克卢维酵母细胞磁分离:壳聚糖包覆磁性纳米颗粒的固定化
摘要本研究研究了壳聚糖包被磁性纳米颗粒(C-MNP)与木霉孢子和马氏克卢维酵母细胞的相互作用。通过Plackett-Burman设计,发现直接影响酵母细胞固定化和磁分离的因素有接种量、C-MNP用量、搅拌速度、作用时间和培养基体积,而对于真菌孢子,温度也是一个影响因素。采用Langmuir和Freundlich模型对吸附等温线进行了数学分析。对于木霉孢子吸附等温线,Langmuir模型线性函数的相关系数最高,吸附量最大为5.00E + 09孢子(C-MNP g−1)。K. marxianus细胞的吸附等温线较好地调整为Freundlich模型,其常数(Kf)估计为2.05E + 08 cells (C-MNP g−1)。这两种系统都可能在生物质的磁分离辅助发酵过程中有新的应用。
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