Exopolysaccharyde production by Cryptococcus laurentii SD7 using molasses and corn steep liquor as substrates

Q3 Agricultural and Biological Sciences
Adriele do Amor Divino Silva, J. Oliveira, M. L. Cazetta
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引用次数: 2

Abstract

Microbial polysaccharides are of great biotechnological and commercial interest and have wide application in the food, cosmetic and medicine industries. Exopolysaccharide (EPS) production by the yeast Cryptococcus laurentii SD7, isolated from fresh water molluscs, was studied using agro-industrial byproducts as substrates in the submerged fermentation. The Central Composite Design (CCD) 23 was used to study the influence of pH, different concentrations on sugarcane molasses and corn steep liquor (CSL), for 48 hours. According to the results, the highest EPS production occurred at the initial pH 5 and at 8.4% concentration of sugarcane molasses, which were statistically significant variable at 10% (p < 0.1). The concentration of CSL had no influence in the studied range, thus, it can be used lowest concentration (0.3%). The time course of EPS production showed that while cell growth peaked within 48 hours, the highest EPS production (6.61 g L-1) occurred at 168 hours, with a productivity of about 0.04 g L-1 h-1. The pH of the medium remained approximately constant throughout the fermentation process. The yeast C. laurentii SD7 showed great potential for EPS production at a low cost and with sustainable substrates.
laurentii隐球菌SD7以糖蜜和玉米浆为底物生产胞外多糖
微生物多糖具有重要的生物技术和商业价值,在食品、化妆品和医药等行业有着广泛的应用。研究了从淡水软体动物中分离的laurenti隐球菌(Cryptococcus laurentii) SD7酵母以农工副产物为底物进行深层发酵生产胞外多糖(EPS)。采用中心组合设计(CCD) 23研究了pH、不同浓度对甘蔗糖蜜和玉米浸泡液(CSL)处理48 h的影响。结果表明,初始pH为5和甘蔗糖蜜浓度为8.4%时EPS产量最高,10%时EPS产量差异有统计学意义(p < 0.1)。CSL的浓度在研究范围内无影响,可采用最低浓度(0.3%)。EPS产量的时间过程表明,细胞在48 h内达到生长高峰,而在168 h时EPS产量最高(6.61 g L-1),产量约为0.04 g L-1 h-1。培养基的pH值在整个发酵过程中大致保持恒定。酵母C. laurentii SD7具有低成本和可持续底物生产EPS的巨大潜力。
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来源期刊
Acta Scientiarum. Biological Sciences
Acta Scientiarum. Biological Sciences Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.00
自引率
0.00%
发文量
45
审稿时长
38 weeks
期刊介绍: The journal publishes original articles in all areas of Biological Sciences, including anatomy, bacteriology, molecular biology, biochemistry, botany, cytology and cell biology, animal behavior, ecology, limnology, embryology, and histology, morpho-physiology, genetics, microbiology, parasitology and zoology.
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