Hydrolyzed VCO Cream Reduces Neutrophil Number and Increases Angiogenesis in Mid Dermal Burn Wound Healing

The Indonesian Biomedical Journal Pub Date : 2023-06-26 DOI:10.18585/inabj.v15i3.2228

Septin Kamilia Patinggi, Y. Bakhtiar, Selamat Budijitno, N. Susilaningsih, U. Bahrudin

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Abstract

BACKGROUND: Researches on plant active substances are conducted to find effective topical drugs for mid dermal burns wound. The 70% hydrolyzed VCO (hVCO) cream has been known to accelerate macroscopic healing of mid dermal burns compared to 30% and 50% hVCO creams. This study was conducted to further investigate the effectiveness of 70% hVCO cream and find the most effective doses of hVCO to heal mid dermal burns based on the macroscopic diameter, neutrophil number and angiogenesis.METHODS: Thirty mid dermal burns-induced male Rattus norvegicus were randomly divided into 6 groups that were either treated with 70% hVCO cream, 100% hVCO cream, or basic cream for 6 or 12 days. Macroscopic appearance of mid dermal burn was documented, and the diameter was calculated. Hematoxylin Eosin (HE) examination was conducted to measure the neutrophil and angiogenesis in mid dermal burn wound.RESULTS: The decrease of wound macroscopic size was found to be significant after 6 and 12 days of treatment (p=0.004 and p<0.001, respectively), with the smallest diameter was found in 70% hVCO group. The lowest number of neutrophils was found in the 70% hVCO group at day-6 (p=0.039) and day-12 (p=0.013). There was a significant increase of angiogenesis at day-12 (p=0.025) with the highest value obtained in the 70% hVCO group.CONCLUSION: The 70% hVCO decreases the wound macroscopic size, decreases neutrophil number, and increases angiogenesis compared to the 100% hVCO. Hence, 70% hVCO isshown to be the most effective dose in accelerating mid dermal burns wound healing andcan be explored further as topical medication.KEYWORDS: hVCO, neutrophil, angiogenesis, wound healing

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水解VCO霜减少中性粒细胞数量，增加真皮烧伤创面愈合中的血管生成

背景:通过对植物活性物质的研究，寻找对真皮烧伤创面有效的外用药物。已知70%水解VCO (hVCO)乳膏与30%和50%水解VCO乳膏相比，可加速真皮中部烧伤的肉眼愈合。本研究进一步考察70% hVCO乳膏的疗效，并根据宏观直径、中性粒细胞数量和血管生成情况，寻找hVCO治疗真皮中烧伤的最有效剂量。方法:将30只雄性褐家鼠随机分为6组，分别用70% hVCO乳膏、100% hVCO乳膏和基础乳膏治疗6、12 d。记录中皮烧伤的肉眼形态，并计算烧伤直径。采用苏木精伊红(HE)检测中皮烧伤创面中性粒细胞及血管新生情况。结果:治疗6 d、12 d后创面肉眼大小明显减小(p=0.004、p<0.001)， 70% hVCO组创面直径最小。70% hVCO组中性粒细胞数量在第6天(p=0.039)和第12天(p=0.013)最低。血管生成在第12天显著增加(p=0.025)， 70% hVCO组达到最高值。结论:与100% hVCO相比，70% hVCO使创面宏观尺寸减小，中性粒细胞数量减少，血管生成增加。因此，70% hVCO被证明是加速真皮烧伤创面愈合的最有效剂量，可以进一步探索作为局部用药。关键词:hVCO，中性粒细胞，血管生成，伤口愈合

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The Indonesian Biomedical Journal

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