The differences in inflammation changes between lymphoid and non lymphoid tissues of mice after i.p. E.coli injection and its effect on renal function

Abstract

Splenic microenvironments are suitable for the definition of immunologic processes, which may different than other non lymphoid organs. Splenic changes have been identified after Leishmania and viral infections, but after bacterial need more study. In kidney, as a non lymphoid organ, the bacterial infection associated with acute kidney injury. Acute kidney injury can lead to chronic kidney disease which can lead to kidney failure. The present work amid to study the differences between bacterial infection stages in lymphoid organ, spleen, and nonlymphoid organ, kidney; and the effect of bacterial infection stage on the renal function. 20 males Balb/c mice were used in this study, aged about 2 months and weight about 24g. 15 mice were injected intraperitoneal with 200μl E.coli, isolated from children have urinary infection, in final concentration (10cell /ml), and 5 mice were injected with only normal saline as control group. The mice were killed in different time after E.coli injection: 24, 48, and 72h. Blood was collected and used in serum creatinine and blood urea determinations. Spleen and kidney were collected, fixed in 10% formalin solution, and processed by standard procedures. The body weights were recorded before sacrifice. The results showed that: significantly higher body weight in infected groups within 24, 48, and 72h compared to zero time and control group, significantly negative correlations between body weight and infection duration, white pulp widening, back off red pulp 10 Saad A. AL-Jashaami et al. size, and activation of germinal center with the time after bacterial injection, infiltration of inflammatory cells and appearing of the focal necrosis of renal epithelial tubules within 48h after bacterial infection and increased after 72h, significantly higher serum creatinine (S.Cr) and blood urea (B.Urea) in the infected groups within 48 and 72h after bacterial injection compared to control group, and significantly positive correlations between S.Cr and B.Urea levels in infected groups and all groups together but not in the control group.