{"title":"The Effect of Vitrification of Oocytes Cumulus Complex Apoptosis of Mice (Mus musculus) to Apoptosis, Rate of Fertilization and Embryo Quality","authors":"W. Widjiati, A. Aulanni’am, V. Hendrawan","doi":"10.25258/IJPCR.V9I3.8314","DOIUrl":null,"url":null,"abstract":"The purpose of this study was to determine the quality of cumulus oocyte complex (COC) after vitrification process\ntoward apotosis of COC, fertilization rate and embryo quality. Frozen occurs during the cold shock that can lead to\nchanges in the molecular level COC. These changes will affect the quality of frozen thawed oocytes after COC. The\nstudy used two groups, There are COC not frozen and frozen COC. Parameter observed were apotosis of blastomere\ncells, fertilization rate and embryo quality. Apoptosis of COC were observed with tunnel apoptec staining, fertilization\nrates were measured based on number of zygotes and embryo quality were observed through number of blastocyst. The\ndata of apotosis of blastomere cells were analyzed by Kruscal Wallis. The result showed that the apoptosis number,\nfertilization rate, and morula number between are significantly (p less than 0.05) between groups. The administration of frozen\nCOC increase number of apoptotic blastomer cells, decreased fertilization rate up to 51.1% and embryo quality up to\n69.2 %. In conclusion, Frozen on COC increased apotosis of COC, decreased both of fertilization rate and embryo\nquality. The embryo Frozen of vitrification is required in the ART technology necessary to add an antioxidant to improve\nthe fertilization rate and embryo quality","PeriodicalId":19889,"journal":{"name":"药学与临床研究","volume":"260 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2017-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"药学与临床研究","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.25258/IJPCR.V9I3.8314","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
The purpose of this study was to determine the quality of cumulus oocyte complex (COC) after vitrification process
toward apotosis of COC, fertilization rate and embryo quality. Frozen occurs during the cold shock that can lead to
changes in the molecular level COC. These changes will affect the quality of frozen thawed oocytes after COC. The
study used two groups, There are COC not frozen and frozen COC. Parameter observed were apotosis of blastomere
cells, fertilization rate and embryo quality. Apoptosis of COC were observed with tunnel apoptec staining, fertilization
rates were measured based on number of zygotes and embryo quality were observed through number of blastocyst. The
data of apotosis of blastomere cells were analyzed by Kruscal Wallis. The result showed that the apoptosis number,
fertilization rate, and morula number between are significantly (p less than 0.05) between groups. The administration of frozen
COC increase number of apoptotic blastomer cells, decreased fertilization rate up to 51.1% and embryo quality up to
69.2 %. In conclusion, Frozen on COC increased apotosis of COC, decreased both of fertilization rate and embryo
quality. The embryo Frozen of vitrification is required in the ART technology necessary to add an antioxidant to improve
the fertilization rate and embryo quality