Typing of Common Dermatophytes by Random Amplification of Polymorphic DNA

Nippon Ishinkin Gakkai Zasshi Pub Date : 1997-07-30 DOI:10.3314/JJMM.38.239

Zhihong Zhong, Ruoyu Li, Dongmei Li, Duanli Wang

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引用次数: 30

Abstract

Seventy-six clinical isolates of dermatophytes and 8 preserved strains of Arthroderma were investigated by random amplification of polymorphic DNA (RAPD) assay to determine the DNA types of common dermatophytes and find any relationships of DNA-based typing with morphology, teleomorphs, geographic origins and sites of human infection. It was found that each species showed a distinct DNA pattern, which can be used as an identification marker. Forty-two isolates of Trichophyton mentagrophytes were classified into 3 main types and intratype polymorphism was revealed with some primers. No simple relationship was found between DNA type and morphology, but the DNA types of strains were closely related to their geographic origins. The RAPD groups of Arthroderma benhamiae, A. vanbreuseghemii, A. gypsea and A. otae were distinctly different, whereas the 42 clinical isolates of T. mentagrophytes shared similar patterns with A. vanbreuseghemii. Among 30 clinical isolates of T. rubrum, 22 showed almost identical RAPD type, while the other 8 strains differed slightly in band patterns. T. rubrum exhibited great differences from A. benhamiae and A. vanbreuseghemii. In conclusion, RAPD provides a stable and reliable means of typing common dermatophytes, and is a powerful tool for dermatophyte identification and epidemiological study.

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多态DNA随机扩增的常见皮肤真菌分型研究

采用随机多态性DNA扩增(RAPD)方法，对76株临床分离的皮癣菌和8株保存的节肢菌进行了DNA分型研究，探讨了常见皮癣菌的DNA分型与人体形态、远形、地理起源和感染地点的关系。结果发现，每个物种都有不同的DNA模式，可以作为鉴定标记。利用引物将42株毛癣菌分为3个主要类型，并分析了其型内多态性。菌株的DNA类型与形态之间没有简单的关系，但菌株的DNA类型与其地理起源密切相关。benhamiae节肢动物、A. vanbreuseghemi、A. gypsea和A. otae的RAPD组有明显差异，而42个临床分离的mentagrophytes与A. vanbreuseghemi具有相似的模式。在30株临床分离的红螺旋体中，22株的RAPD型基本一致，其余8株的RAPD型略有差异。红绒田鼠与benhamiae田鼠和vanbreuseghemi田鼠表现出很大的差异。总之，RAPD提供了一种稳定可靠的常见皮肤病菌分型方法，是皮肤病菌鉴定和流行病学研究的有力工具。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Nippon Ishinkin Gakkai Zasshi

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