Quantitative standardization of pharmacologically active components from antiplasmodial plant Solanum nudum Dunal (wild and in vitro)

IF 0.7 4区医学 Q4 INTEGRATIVE & COMPLEMENTARY MEDICINE

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas Pub Date : 2022-01-01 DOI:10.37360/blacpma.22.21.1.02

A. M. Mesa-Vanegas, E. J. Naranjo-Gomez, Felipe Cardona, Lucia Atehortua-Garces, S. Blair-Trujillo

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Abstract

Solanum nudum Dunal (Solanaceae) is most commonly known and used by the population of the colombian Pacific coast as an antimalarial treatment. This article study into optimization and quantitative analysis of compounds steroidal over time of development of this species when grown in vitro and wild. A new steroidal compound named SN6 was elucidated by NMR and a new method of quantification of seven steroidal compounds (Diosgenone DONA and six steroids SNs) using HPLC-DAD-MS in extracts of cultures in vitro and wild was investigated. Biology activity of extracts was found to a range of antiplasmodial activity in FCB2 and NF-54 with inhibitory concentration (IC50) between (17.04 -100 μg/mL) and cytotoxicity in U-937 of CC50 (7.18 -104.7 μg/mL). This method creates the basis for the detection of seven sterols antiplasmodial present in extracts from S. nudum plant as a quality parameter in the control and expression of phytochemicals.

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抗疟原虫植物龙葵(野生和离体)药理活性成分的定量标准

龙葵(龙葵科)是哥伦比亚太平洋沿岸最常见的一种抗疟疾药物。本文研究了该物种在离体生长和野生生长时甾体化合物随时间变化的优化和定量分析。通过核磁共振鉴定了一种新的甾体化合物SN6，并研究了用HPLC-DAD-MS定量7种甾体化合物(薯蓣皂苷酮DONA和6种甾体SNs)的新方法。对FCB2和NF-54的抑制浓度(IC50)在17.04 ~ 100 μg/mL之间，对CC50的U-937的细胞毒性为7.18 ~ 104.7 μg/mL。该方法可作为植物化学物质控制和表达的质量参数，为裸参提取物中7种抗疟原虫甾醇的检测奠定基础。

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来源期刊

Boletin Latinoamericano y del Caribe de Plantas Medicinales y Aromaticas PHARMACOLOGY & PHARMACY-

CiteScore

1.30

自引率

14.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas (BLACPMA), [Latin American and Caribbean Bulletin of Medicinal and Aromatic Plants]; currently edited by the publishing house MS-Editions, is a bi-monthly international publication that publishes original peerreviewed research in the field of medicinal and aromatic plants, with nearly 20 years of experience. BLACPMA is a scientific journal that publishes two types of articles: Reviews (only in English) and Original Articles (Spanish or English), its main lines of action being agronomy, anthropology and ethnobotany, industrial applications, botany, quality and standardization, ecology and biodiversity, pharmacology, phytochemistry, pharmacognosy, regulatory and legislative aspects. While all areas of medicinal plants are welcome and the experimental approaches used can be broad and interdisciplinary; other areas of research that are not mentioned depend on the Editorial Committee for their acceptance.