Application of Raman Spectroscopy to the Biooxidation Analysis of Sulfide Minerals

J. V. García-Meza, R. H. Lara, H. Navarro‐Contreras
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引用次数: 8

Abstract

We report the application of confocal laser scanning microscopy CLSM and Raman spectroscopy on the (bio)chemical oxidation of pyrite and chalcopyrite, in order to understand how surface sulfur species (S𝑛2−/S0) affects biofilm evolution during mineral colonization by Acidithiobacillus thiooxidans. We found that cells attachment occurs as cells clusters and monolayered biofilms within the first 12 h. Longer times resulted in the formation of micro- and macrocolonies with variable cell density and higher epifluorescence signal of the extracellular polymeric substances (EPS), indicating double dynamic activity of A. thiooxidans: sulfur biooxidation and biofilm formation. Raman spectra indicated S𝑛2−/S0 consumption modification during biofilm evolution. Hence, cell density increase was primarily associated with the presence of S0; the presence of refractory sulfur species on the mineral surfaces does not to affect biofilm evolution. The EPS of the biofilms was mainly composed of extracellular hydrophobic compounds (vr. gr. lipids) and a minor content of hydrophilic exopolysaccharides, suggesting a hydrophobic interaction between attached cells and the altered pyrite and chalcopyrite.
拉曼光谱在硫化物矿物生物氧化分析中的应用
我们报道了应用激光共聚焦扫描显微镜CLSM和拉曼光谱研究黄铁矿和黄铜矿的(生物)化学氧化,以了解表面硫(S𝑛2−/S0)如何影响硫氧化硫硫杆菌在矿物定植过程中的生物膜演化。我们发现细胞在最初的12小时内以细胞簇和单层生物膜的形式附着。较长的时间导致细胞密度变化的微集落和大集落的形成,细胞外聚合物质(EPS)的表观荧光信号较高,表明硫氧化铁具有硫生物氧化和生物膜形成的双重动态活性。拉曼光谱表明S𝑛2−/S0消耗在生物膜演化过程中发生了改变。因此,细胞密度的增加主要与S0的存在有关;矿物表面难熔硫物质的存在不影响生物膜的演化。生物膜的EPS主要由胞外疏水化合物(vr)组成。脂质)和少量亲水胞外多糖的含量,表明附着细胞与改变的黄铁矿和黄铜矿之间存在疏水相互作用。
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