IDENTIFIKASI LACTOBACILLUS GASSERI DARI ASI: KARAKTERISASI DAN PENGUJIAN BIOKIMIA

Agricore: Jurnal Agribisnis dan Sosial Ekonomi Pertanian Unpad Pub Date : 2019-12-03 DOI:10.24198/agricore.v2i2.21313

I. Hanidah, Dimas Erlangga, Debby M Sumantri, Willa Kusumah Wardani

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引用次数: 1

Abstract

ABSTRAK ASI dari ibu yang sehat memiliki potensi yang besar mengandung bakteri probiotik spesies Lactobacillus. Hasil penelitian menunjukkan bahwa 20% dari sampel lima ASI Ibu menyusui usia 12 – 60 hari setelah melahirkan mengandung bakteri probiotik Lactobacillus gasseri dengan karakteristik morfologi: gram positif, katalase negative, non motil, anaerob, bentuk koloni bulat dengan permukaan cembung, warna koloni putih susu agak krem, tekstur koloni agak keras, koloni tumbuh dibagian tengah media agar (anaerob), sel berbentuk basil dengan ukuran sel 2,0 µm. sedangkan uji biokimia menunjukkan bahwa isolate L. glasseri terseleksi mampu menguraikan: D-Cellobiose, Saccharose, Maltotriose, Phosphatase, Leucine Arylamidase, Tryosine Arylamidase, Arbutin, Esculin hydrolysis, Ala-Phe-Pro_Arylamidase, N-Acetyl-D-Glucosamine, Phenylalanine Arylamidase, D-Glukose, 5-Bromo-4-chloro-3-indoxyl-beta-glucoside, L-Proline Arylamidase, D-Mannose, Arginine GP, D-Maltose. Kata kunci: Lactobacillus gasseri, probiotik, ASI. ABSTRACT Breast milk from healthy mothers has a great potential to contain the probiotic bacteria of the Lactobacillus species. The results showed that 20% of the five samples of breast milk, at days 12 to 60 after birth contained probiotic bacteria of Lactobacillus gasseri with morphology of bacterial: gram-positive, catalase- negative, non-motile, anaerob, rounded colony shape with convex surface, the color colony of slightly creamy, rather hard colony texture, anaerobic, basil-shaped cells with a cell size of 2.0 μm. Biochemical tests show that selected L. glasseri isolates are able to decipher: D-Cellobiose, Saccharose, Maltotriose, Phosphatase, Leucine Arylamidase, Tryosine Arylamidase, Arbutin, Esculin hydrolysis, Ala-Phe-Pro_Arylamidase, N-Acetyl-D-Glucosamine, Phenylalanine Arylamidase, D-Glukose, 5-Bromo-4-chloro-3-indoxyl-beta-glucoside, L-Proline Arylamidase, D-Mannose, Arginine GP, D-Maltose. Keywords: Lactobacillus gasseri, probiotic, breast milk

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母乳拉西杆菌:生物化学特征和测试

来自健康母亲的抽象母乳具有一种菌生菌菌的巨大潜力。母乳样本的研究结果表明,20%五产后哺乳期母亲12岁——60天含有益生菌的细菌Lactobacillus gasseri用积极的形态学特征:克katalase消极的、非基序与凸表面氧,形状圆的殖民地,乳白色的殖民地有点奶油的颜色,质地有点固执的殖民地,在媒体中成长,使殖民地(氧),细胞细胞形状尺寸的巴兹尔2.0µm。而生物化学测试表明，每种合成物的合成物具有D-Cellobiose, Saccharose, multotriose，磷酸酶，磷酸酶，拟胺胺，酯-苯丙胺关键词:味丝菌、益生菌、母乳。营养不良母亲的乳汁具有巨大的潜力来控制菌系。results那里那20%》五样本的胸中之妙,在之后的60天12到分娩有趣益生菌的细菌Lactobacillus gasseri with morphology of bacterial: gram-positive catalase -不行,non-motile、氧rounded殖民地和凸形状,地面颜色殖民地》,有点奶油很硬殖民地纹理、anaerobic basil-shaped细胞与a细胞大小的2.0μm。生物化学测试显示，这些受欢迎的L- cellobiose可以分解:d - cellabiose, Saccharose, multotriose，磷酸酶，磷酸酶，拟胺，酯-苯胺Keywords: Lactobacillus gas系里，probiotic, breast milk

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Agricore: Jurnal Agribisnis dan Sosial Ekonomi Pertanian Unpad

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