Synthesis of Glucosylxyluloside Using Sucrose Phosphorylase

Abstract

Synthesis of glucosylxyluloside (Glc-Xul) by the action of sucrose phosphorylase [EC 2.4. 1.7] from Leuconostoc mesenteroides was studied . In the transfer reaction using equimolar amount of a-D-glucose-l-phosphate (G-1-P) and D-xylulose, about 25% of D-xylulose was converted into Glc-Xul, whereas only 17% of D-fructose was converted into sucrose when D-fructose was used instead of D-xylulose . In the transfer reaction using equimolar amount of sucrose andD-xylulose, more than 60% of D-xylulose was converted into Glc -Xul . It was found that more than 80% of added D-xylulose was converted into Glc-Xul when the molar ratio of sucrose to D-xylulose was increased to 3.