Reappraisal of Anoxic Spreading Depolarization as a Terminal Event in Brain Slices

Abstract

In the present study we explored neuronal survival during oxygen-glucose deprivation (OGD) in slices of the barrel cortex using whole-cell patch clamp recordings and concomitant extracellular recordings of local field potential (LFP) ,and optical intrinsic signal (OIS). To this aim we used two types of neuronal survival assessment. In the first approach, we performed continuous recordings from a single neuron starting from control conditions and then through OGD and reperfusion. In the second approach, we sampled cells using short-term patch-clamp recordings in control conditions and during reperfusion following OGD of various durations (from 1 to 60 min).