Tomato (Lycopersicon esculentum) Extracts Elicit Anti-Hepatotoxic Effects on Acetaminophen-Induced Liver Injury in Albino Wistar Rat

Technological Innovation in Pharmaceutical Research Vol. 10 Pub Date : 2021-08-03 DOI:10.9734/bpi/tipr/v10/11084d

I. Uchendu, C. Agu, O. C. Orji, Ebuka Bitrus Nnedu, C. Arinze, A. Uchenna, U. Okongwu

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Abstract

Introduction: It has been extensively reported that drug-overdose is the leading cause of liver injury in the world today. Diets rich in natural antioxidants have been observed to offer substantial remedy to drug-induced organ injuries. Objective: The aim of this study was to investigate the hepatoprotective effects of tomato extract against Acetaminophen-induced acute hepatotoxicity in rats. Methods: Phytochemical analyses were performed. A total of 24 albino rats weighing (110±10 g) were randomly allocated to four groups (A-D), with six rats per group. Group A was the normal control and received no treatment. Group B was the negative control and received a single dose of acetaminophen (750 mg/kg, i.p) only. Group C served as test group and received a single dose of acetaminophen (750 mg/kg, i.p.) before treatment with tomato extract (30 mg/kg, oral) for 14 consecutive days. Group D received simultaneous administration of tomato extract (30 mg/kg, oral) and acetaminophen (750 mg/kg, i.p) for 14days. Results: The single dose of Acetaminophen caused liver cell injuries with significant increase in the levels of the liver enzymes: AST (67.67±11.41U/L); ALT (46.33±10.59U/L) and ALP (223.70±23.31U/L) in rats in negative control when compared with normal (p<0.05 or p<0.01). The daily administration of the tomato extract was able to attenuate the acetaminophen-induced hepatotoxicity on the liver enzyme marker levels: In Group C: AST (23.00± 3.61U/L, P<0.01); ALT (17.67± 3.48U/L, P<0.05) and ALP (121.30±8.11U/L, P<0.01). In Group D, AST (26.67±2.91U/L, P<0.01); ALT (18.67±1.76 U/L, P<0.05) and ALP (124.72±9.33U/L, P<0.01) when compared with negative control group. The histological results also revealed no significant liver injury in the groups that received tomato extract when compared with the normal control. Conclusion: Tomato extract possesses hepatoprotective ability against acetaminophen-induced liver injury.

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番茄提取物对对乙酰氨基酚诱导的白化Wistar大鼠肝损伤的抗肝毒作用

导读:据广泛报道，药物过量是当今世界肝损伤的主要原因。富含天然抗氧化剂的饮食已被观察到对药物引起的器官损伤提供实质性的补救措施。目的:研究番茄提取物对对乙酰氨基酚所致大鼠急性肝毒性的保护作用。方法:进行植物化学分析。选取体重(110±10 g)的白化大鼠24只，随机分为4组(A- d)，每组6只。A组为正常对照组，不进行任何治疗。B组为阴性对照，只给予单剂量对乙酰氨基酚(750 mg/kg, i.p)。C组为试验组，在给予番茄提取物(30 mg/kg，口服)治疗前，给予单剂量对乙酰氨基酚(750 mg/kg，每日1次)，连续14 d。D组患者同时给予番茄提取物(30 mg/kg，口服)和对乙酰氨基酚(750 mg/kg，每日1次)，疗程14 D。结果:单剂量对乙酰氨基酚引起肝细胞损伤，肝酶AST水平显著升高(67.67±11.41U/L);阴性对照组大鼠ALT(46.33±10.59U/L)、ALP(223.70±23.31U/L)与正常大鼠比较差异有统计学意义(p<0.05或p<0.01)。每日给药番茄提取物可减轻对乙酰氨基酚引起的肝毒性，其肝酶标志物水平为:C组:AST(23.00±3.61U/L, P<0.01);ALT(17.67±3.48 u / L, P < 0.05)和高山(121.30±8.11 u / L, P < 0.01)。D组AST(26.67±2.91U/L, P<0.01);与阴性对照组比较，ALT(18.67±1.76 U/L, P<0.05)、ALP(124.72±9.33U/L, P<0.01)。组织学结果也显示，与正常对照组相比，接受番茄提取物的组没有明显的肝损伤。结论:番茄提取物对对乙酰氨基酚所致肝损伤具有保护肝的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Technological Innovation in Pharmaceutical Research Vol. 10

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