Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics

Sameena Yousuf, I. V. Enoch
{"title":"Binding of the Bi (III) Complex of Naringin with β-Cyclodextrin/Calf Thymus DNA: Absorption and Fluorescence Characteristics","authors":"Sameena Yousuf, I. V. Enoch","doi":"10.1155/2014/562160","DOIUrl":null,"url":null,"abstract":"Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β-CD was 1 : 1 with a binding constant of 5.18 × 102 mol−1 dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β-CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.29 × 105 mol−1 dm3 and 6.89 × 104 mol−1 dm3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.25 × 104 mol−1 dm3 and 5.10 × 103 mol−1 dm3, respectively. The lowering of the binding affinity and the were observed for the interaction of Narb with ctDNA in the presence of β-CD.","PeriodicalId":14329,"journal":{"name":"International Journal of Spectroscopy","volume":"48 1","pages":"1-8"},"PeriodicalIF":0.0000,"publicationDate":"2014-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Spectroscopy","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1155/2014/562160","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1

Abstract

Naringin-Bi (III) complex (Narb) was prepared and analysed by UV-Visible absorption and fluorescence measurements. The inclusion complex of Narb with β-Cyclodextrin (β-CD) was characterized by the UV-Visible absorption, Infrared, scanning dlectron microscopic, and X-ray diffractometric techniques. The stoichiometry of the inclusion complex of Narb with β-CD was 1 : 1 with a binding constant of 5.18 × 102 mol−1 dm3. The interaction of Narb with Calf Thymus DNA (ctDNA) was investigated in the presence and the absence of β-CD. The binding constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.29 × 105 mol−1 dm3 and 6.89 × 104 mol−1 dm3, respectively. The Stern-Volmer constants for the interaction of Narb with ctDNA in the absence and the presence of β-CD were 1.25 × 104 mol−1 dm3 and 5.10 × 103 mol−1 dm3, respectively. The lowering of the binding affinity and the were observed for the interaction of Narb with ctDNA in the presence of β-CD.
柚皮苷Bi (III)配合物与β-环糊精/小牛胸腺DNA的结合:吸收和荧光特性
制备了柚皮苷-碧(III)配合物(Narb),并对其进行了紫外可见吸收和荧光分析。通过紫外可见吸收、红外、扫描电镜和x射线衍射等技术对Narb与β-环糊精(β-CD)的包合物进行了表征。Narb与β-CD包合物的化学计量比为1:1,结合常数为5.18 × 102 mol−1 dm3。在存在和不存在β-CD的情况下,研究了Narb与小牛胸腺DNA (ctDNA)的相互作用。在β-CD不存在和β-CD存在的情况下,Narb与ctDNA的结合常数分别为1.29 × 105 mol−1 dm3和6.89 × 104 mol−1 dm3。在β-CD不存在和β-CD存在的情况下,Narb与ctDNA相互作用的Stern-Volmer常数分别为1.25 × 104 mol−1 dm3和5.10 × 103 mol−1 dm3。在β-CD存在的情况下,Narb与ctDNA相互作用的结合亲和力降低。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信