{"title":"Ginkgo biloba extract EGB761 improved anti-heat stress responses in chickens in vivo via regulation of heat-shock protein expression and distribution","authors":"Xiaohui Zhang, Miao Zhang, E. Bao","doi":"10.3906/vet-1910-79","DOIUrl":null,"url":null,"abstract":"Heat stress is a lethal threat to chickens. This study investigated the protective effect of a Ginkgo biloba extract EGB761 against heat stress in chickens in vivo. A total of 200 one-day-old hens were separated randomly into control Con , heat stress HS , 0.1% EGB administration and heat stress 0.1% EGB+HS , 0.3% EGB administration and heat stress 0.3% EGB+HS , and 0.6% EGB administration and heat stress 0.6% EGB+HS groups. After EGB761 administration for 45 days, ten chickens were selected randomly, assigned to one of the designated groups, and exposed to a one-time heat stress condition of 38 ± 1 °C for 3 h. The results showed that EGB761 administration improved the growth performance and physiological condition of the chickens, reducing feeding-related mortality. EGB761 lowered the histological scores of the heart, liver, and duodenum in the heat-stressed chickens. EGB761 significantly reduced the transcription and translation levels of Hsp27, Hsp70, and Hsp90 in these organs. EGB761 might promote the transformation of Hsps in the heart, increase the nuclear translocation of Hsp27 and Hsp90 in hepatocytes, and regulate the accumulation of Hsp27 and Hsp70 in intestinal villi. Therefore, EGB761 administration is a potential method for protecting chickens from acute heat stress damage.","PeriodicalId":23357,"journal":{"name":"Turkish Journal of Veterinary & Animal Sciences","volume":"5 1","pages":"191-200"},"PeriodicalIF":0.5000,"publicationDate":"2020-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Turkish Journal of Veterinary & Animal Sciences","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.3906/vet-1910-79","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Heat stress is a lethal threat to chickens. This study investigated the protective effect of a Ginkgo biloba extract EGB761 against heat stress in chickens in vivo. A total of 200 one-day-old hens were separated randomly into control Con , heat stress HS , 0.1% EGB administration and heat stress 0.1% EGB+HS , 0.3% EGB administration and heat stress 0.3% EGB+HS , and 0.6% EGB administration and heat stress 0.6% EGB+HS groups. After EGB761 administration for 45 days, ten chickens were selected randomly, assigned to one of the designated groups, and exposed to a one-time heat stress condition of 38 ± 1 °C for 3 h. The results showed that EGB761 administration improved the growth performance and physiological condition of the chickens, reducing feeding-related mortality. EGB761 lowered the histological scores of the heart, liver, and duodenum in the heat-stressed chickens. EGB761 significantly reduced the transcription and translation levels of Hsp27, Hsp70, and Hsp90 in these organs. EGB761 might promote the transformation of Hsps in the heart, increase the nuclear translocation of Hsp27 and Hsp90 in hepatocytes, and regulate the accumulation of Hsp27 and Hsp70 in intestinal villi. Therefore, EGB761 administration is a potential method for protecting chickens from acute heat stress damage.
期刊介绍:
The Turkish Journal of Veterinary and Animal Sciences is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK).
Accepts English-language manuscripts on all aspects of veterinary medicine and animal sciences.
Contribution is open to researchers of all nationalities.
Original research articles, review articles, short communications, case reports, and letters to the editor are welcome.
Manuscripts related to economically important large and small farm animals, poultry, equine species, aquatic species, and bees, as well as companion animals such as dogs, cats, and cage birds, are particularly welcome.
Contributions related to laboratory animals are only accepted for publication with the understanding that the subject is crucial for veterinary medicine and animal science.
Manuscripts written on the subjects of basic sciences and clinical sciences related to veterinary medicine, nutrition, and nutritional diseases, as well as the breeding and husbandry of the above-mentioned animals and the hygiene and technology of food of animal origin, have priority for publication in the journal.
A manuscript suggesting that animals have been subjected to adverse, stressful, or harsh conditions or treatment will not be processed for publication unless it has been approved by an institutional animal care committee or the equivalent thereof.
The editor and the peer reviewers reserve the right to reject papers on ethical grounds when, in their opinion, the severity of experimental procedures to which animals are subjected is not justified by the scientific value or originality of the information being sought by the author(s).