Towards a Widening of Romanian Nuclear-Stock Plum Varieties

L. Zagrai, I. Zagrai, G. Guzu, C. Moldovan
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Abstract

To reduce economic losses caused by viruses and phytoplasmas, one of the main preventive measures is to use 'certified' plant material. A nuclear-stock plum collection under insect-proof house is conserved at FRDS Bistrița and consists in twenty-one varieties and one rootstock used for stone fruits propagation. A widening of Romanian plum varieties at Pre-basic category is required to create the opportunity to establish a larger national valuable nuclear-stock collection and to secure the prerequisite for a good transfer of Romanian plum breeding results to the practical application. Plants belonging to other six Romanian plum varieties (Diana, Silvia, Tita, Pescăruș, Gras românesc and Tuleu dulce) were monitored in the field along two consecutive years for potential viral symptoms and tested by serological and molecular tools according EPPO standards. Selected trees of each variety were grafted onto virus-free Saint Julien rootstocks, into insect-proof house, and retested. Three grafted plants from each of the six varieties were chosen to become Candidate Pre-basic material and they were biological indexed onto virus free plants of GF 305. Thus, candidate Pre-basic material, the precursor for Pre-basic category, was established to other six valuable Romanian plum varieties.
罗马尼亚核砧木李子品种的扩大研究
为了减少病毒和植物原体造成的经济损失,主要的预防措施之一是使用“认证”的植物材料。在FRDS Bistrița保存了一个防虫房下的核砧木李子,包括21个品种和1个用于核果繁殖的砧木。罗马尼亚李子品种的扩大需要在前基础类别创造机会,建立一个更大的国家有价值的核储备,并确保罗马尼亚李子育种成果向实际应用的良好转移的先决条件。另外6个罗马尼亚李子品种(Diana、Silvia、Tita、pesccreatrucu、Gras rom nesc和Tuleu dulce)连续两年在田间监测潜在的病毒症状,并根据EPPO标准通过血清学和分子工具进行检测。选择每个品种的树木嫁接到无病毒的圣朱利安砧木上,进入防虫房,并重新测试。从6个品种中各选择3个嫁接植株作为候选Pre-basic材料,并与GF - 305脱毒植株进行生物学标记。由此,确定了候选的Pre-basic材料,即Pre-basic类别的前体,与其他6个有价值的罗马尼亚李子品种相比较。
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