Cytotoxic and Apoptotic Effects of Vanadyl Sulfate on MCF-7 Breast Cancer Cell Line.

IF 3.9 2区经济学 Q1 Economics, Econometrics and Finance

Journal of Financial and Quantitative Analysis Pub Date : 2023-06-21 eCollection Date: 2023-01-01 DOI:10.31661/gmj.v12i.3050

Maryam Dehdashti, Zahra Abbasy, Hamid Zaferani Arani, Hesam Adin Atashi, Seyed Alireza Salimi-Tabatabaee, Afsaneh Ghasemi, Zhila Fereidouni, Hadi Zare Marzouni, Habib Zakeri, Seyed Abbas Mirmalek

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引用次数: 0

Abstract

Background: Breast cancer (BC) is the major cause of cancer-related death in women. Some studies have indicated the cytotoxic effects of vanadyl oxide sulfate (VOSO4). This study aimed to evaluate the anti-cancer effect of VOSO4 in the treatment of MCF-7 cell lines.

Materials and methods: The MCF-7 cell line was treated with different concentrations of VOSO4 for 24 and 48 hours. Cell death was measured using the MTT assay. The cell apoptosis rate was measured using Annexin V/Propidium Iodide assay through flow cytometry. Also, the expression levels of p53, P21, Caspase8, superoxide dismutase type 1 (SOD1), Sod2, and Bcl2 mRNAs were assessed, and Western blotting was performed for Sod1 protein.

Results: The results showed that the half-maximal inhibitory concentration (IC50) for VOSO4 was 25 and 20 μg/ml for 24 and 48 hours, respectively. Indeed, VOSO4 has dose-dependent cytotoxic effects on the MCF-7. Also, after exposure to VOSO4 for 24 hours, cell apoptosis reached 52% compared with untreated cells. Moreover, after 24 hours of exposure to VOSO4 with IC50 concentration, the expression of p53, P21, Caspase8, Sod1, and Sod2 mRNAs increased (P0.05), and the expression of Bcl2 mRNA was decreased (P0.05). Also, the Western blotting revealed Sod1 protein level markedly increased following exposure to VOSO4 (P0.05).

Conclusion: Our results demonstrated that VOSO4 has an apoptotic and cytotoxic effect on BC cells. Therefore, it could be considered a complementary agent for the medical treatment of patients with BC.

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硫酸香草酯对 MCF-7 乳腺癌细胞株的细胞毒性和凋亡效应

背景：乳腺癌（BC）是女性因癌症死亡的主要原因。一些研究表明，硫酸氧香草酯（VOSO4）具有细胞毒性作用。本研究旨在评估 VOSO4 治疗 MCF-7 细胞株的抗癌效果：用不同浓度的 VOSO4 处理 MCF-7 细胞株 24 小时和 48 小时。采用 MTT 法检测细胞死亡。通过流式细胞仪，使用 Annexin V/Propidium Iodide 检测法测量细胞凋亡率。此外，还评估了 p53、P21、Caspase8、1 型超氧化物歧化酶（SOD1）、Sod2 和 Bcl2 mRNA 的表达水平，并对 Sod1 蛋白进行了 Western 印迹：结果表明，在24小时和48小时内，VOSO4的半最大抑制浓度（IC50）分别为25和20微克/毫升。事实上，VOSO4 对 MCF-7 具有剂量依赖性的细胞毒性作用。此外，与未处理的细胞相比，暴露于 VOSO4 24 小时后，细胞凋亡率达到 52%。此外，暴露于 IC50 浓度的 VOSO4 24 小时后，p53、P21、Caspase8、Sod1 和 Sod2 mRNA 的表达量增加（P0.05），而 Bcl2 mRNA 的表达量减少（P0.05）。此外，Western 印迹显示，暴露于 VOSO4 后，Sod1 蛋白水平明显升高（P0.05）：我们的研究结果表明，VOSO4 对 BC 细胞具有凋亡和细胞毒性作用。结论：我们的研究结果表明，VOSO4 对 BC 细胞具有凋亡和细胞毒性作用，因此可被视为 BC 患者药物治疗的辅助药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Financial and Quantitative Analysis Multiple-

CiteScore

6.60

自引率

5.10%

发文量

131

期刊介绍： The Journal of Financial and Quantitative Analysis (JFQA) publishes theoretical and empirical research in financial economics. Topics include corporate finance, investments, capital and security markets, and quantitative methods of particular relevance to financial researchers. With a circulation of 3000 libraries, firms, and individuals in 70 nations, the JFQA serves an international community of sophisticated finance scholars—academics and practitioners alike. The JFQA prints less than 10% of the more than 600 unsolicited manuscripts submitted annually. An intensive blind review process and exacting editorial standards contribute to the JFQA’s reputation as a top finance journal.