Effect of Zinc Oxide nanoparticle on physiological characteristics, rosmarinic acid production and expression of TAT and 4-Cl genes in Lemongrass (Melissa officinalis L.)

Abstract

Lemongrass, Tyrosine aminotransferase, Rosmarinic acid, Coumarate CoA ligase, Zinc oxide nanoparticles Aim: The aim of this study was to investigate the effect of Zinc oxide nanoparticles on growth and physiological characteristics, rosmarinic acid production and the expression of key genes in the biosynthetic pathway of this compound in lemongrass. Material and Methods: In this study, 30-day-old seedlings of lemongrass were treated with concentrations of 0, 0.06 and 0.12 mg/l Zinc oxide nanoparticles, and then growth parameters, photosynthetic pigments, proline, Glycine betaine, protein, activity of antioxidant enzymes and production of rosmarinic acid were examined. Also, the expression of key genes in the rosmarinic acid biosynthetic pathway was examined by real-time PCR. Results: The results indicate that Zinc oxide nanoparticles treatment increased chlorophyll photosynthetic pigments, carotenoids and antioxidant activity of this plant. Also, the highest amount of proline and glycine betaine was obtained at a concentration of 12 mg/L of this treatment. Zinc oxide nanoparticles also increased the expression of rosmarinic acid biosynthetic pathway genes (TAT and 4-Cl) and thus increased this compound. Conclusion: Based on the results of this study, Zinc oxide nanoparticles can affect the growth and physiological stages of lemongrass and therefore it can be used to increase the production of rosmarinic acid. هرود ،تفاب و لولس 13 هرامش ، 1 لاس ، 1401 تاحفص ، 56 ات 70