Cryptic Cyanobacterial Diversity in the Giant Cave (Trieste, Italy): The New Genus Timaviella (Leptolyngbyaceae)

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2017-11-21 DOI:10.7872/crya/v38.iss4.2017.285

Katia Sciuto, E. Moschin, I. Moro

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引用次数: 31

Abstract

Abstract The microflora of hypogean environments has been studied increasingly worldwide. However, some sites have hardly been examined or not studied at all; this is the case for the Giant Cave, a Karst show cave located near Trieste, Italy. In the present study we began characterizing the Giant Cave Lampenflora by using a polyphasic approach, focusing, in particular, on three Leptolyngbya-like strains named GR2, GR4, and GR13. Light and electron microscopic observations were carried out and the water-soluble pigment composition was analysed. Phylogenetic reconstruction, based on the 16S rRNA gene and the 16S-23S ITS region, was performed to better understand the taxonomic position of these strains, complemented by 16S-23S ITS secondary structure analysis. Ecological and geographical data for the investigated strains and for the other cyanobacterial strains grouping with them in the phylogenetic reconstructions were also considered. Based on the results, strain GR2 was ascribed to the species Heteroleibleinia purpurascens (Hansgirg) Anagnostidis & Komárek; strains GR4 and GR13 were attributed to a new genus of the family Leptolyngbyaceae, Timaviella Sciuto & Moro, gen. nov., and represented two distinct species: Timaviella circinata Sciuto & Moro and Timaviella karstica Sciuto & Moro.

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巨型洞穴(意大利的里雅斯特)的隐藻多样性:Timaviella新属(细藻科)

摘要:国内外对低温环境微生物区系的研究日益深入。然而，有些地点几乎没有被检查或根本没有被研究;这就是位于意大利的里雅斯特附近的一个喀斯特洞穴。在本研究中，我们开始使用多相方法来表征巨洞蓝藻，特别关注三种被命名为GR2, GR4和GR13的lepolylynbya样菌株。进行了光镜和电镜观察，并对水溶性色素组成进行了分析。基于16S rRNA基因和16S- 23s ITS区域进行系统发育重建，以更好地了解这些菌株的分类位置，并结合16S- 23s ITS二级结构分析。研究菌株的生态和地理数据以及与之类群的其他蓝藻菌株在系统发育重建中也进行了考虑。根据上述结果，菌株GR2归属于紫癜异白菌(Hansgirg) Anagnostidis & Komárek;菌株GR4和GR13归属于麻麻科新属Timaviella Sciuto & Moro, gen. nov.，代表两个不同的种:circinata Timaviella Sciuto & Moro和karstica Timaviella Sciuto & Moro。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.