SCoT molecular markers are efficient in genetic fingerprinting of pomegranate (Punica granatum L.) cultivars

IF 2.1 4区生物学 Q2 Agricultural and Biological Sciences

Caryologia Pub Date : 2022-09-21 DOI:10.36253/caryologia-1567

Shiva Shahsavari, Z. Noormohammadi, M. Sheidai, F. Farahani, Mohammad Reza Vazifeshenas

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引用次数: 0

Abstract

The pomegranate is an economically important fruit plant species which has been utilized since ancient time as a source of food and medicine by mankind. This plant although is cultivated in certain geographical regions, but its fruits are imported and sold throughout the world. Iran is the center of origin for pomegranate and contains huge number of known cultivars. However, genetic studied on these cultivars are very limited and much detailed information has to be produced for better hybridization and breeding tasks in the country. A fingerprinting study was performed on 178 Punica trees in 47 known cultivars by using SCoT molecular markers. We obtained 61 SCoT bands/ loci which were used for genetic diversity analyses and grouping of the cultivars. A low genetic variability was obtained within and among Punica cultivars, but as revealed by AMOVA, this was quiet enough to produce significant genetic difference among them. DAPC analysis revealed a trace of genetic admixture among the cultivars either due to gene flow or as a result of common ancestral shared alleles. Discriminating SCoT loci may be used in germplasm evaluation of Punica. The genetic difference of these cultivars can be utilized for hybridization and breeding programs.

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SCoT分子标记是一种高效的石榴品种遗传指纹分析方法

石榴是一种经济上重要的水果植物，自古以来就被人类用作食物和药物的来源。这种植物虽然只在某些地理区域种植，但其果实却进口并销往世界各地。伊朗是石榴的原产地中心，拥有大量的已知品种。然而，对这些品种的遗传研究非常有限，必须提供更详细的信息，以便在该国更好地进行杂交和育种任务。利用SCoT分子标记对47个已知品种的178棵Punica树进行了指纹图谱分析。我们获得61个SCoT条带/位点，用于品种的遗传多样性分析和分类。品种间和品种内部的遗传变异性较低，但AMOVA分析表明，这一差异足以产生显著的遗传差异。DAPC分析显示，由于基因流动或共同祖先共有等位基因，品种间存在遗传混合的痕迹。鉴别SCoT位点可用于Punica种质资源评价。这些品种的遗传差异可用于杂交和育种计划。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Caryologia 生物-遗传学

CiteScore

1.60

自引率

23.80%

发文量

审稿时长

12 months

期刊介绍： Caryologia is devoted to the publication of original papers, and occasionally of reviews, about plant, animal and human karyological, cytological, cytogenetic, embryological and ultrastructural studies. Articles about the structure, the organization and the biological events relating to DNA and chromatin organization in eukaryotic cells are considered. Caryologia has a strong tradition in plant and animal cytosystematics and in cytotoxicology. Bioinformatics articles may be considered, but only if they have an emphasis on the relationship between the nucleus and cytoplasm and/or the structural organization of the eukaryotic cell.