Generation of a reporter-null allele of Ppap2b/Lpp3and its expression during embryogenesis.

D. Escalante-Alcalde, Sara Morales, C. Stewart
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引用次数: 18

Abstract

Our knowledge of how bioactive lipids participate during development has been limited principally due to the difficulties of working with lipids. The availability of some of these lipids is regulated by the Lipid phosphate phosphatases (LPPs). The targeted inactivation of Ppap2b, which codes for the isoenzyme Lpp3, has profound developmental defects. Lpp3 deficient embryos die around E9.5 due to extraembryonic vascular defects, making difficult to analyze its participation in later stages of mouse development. To gain some predictive information regarding the possible participation of Lpp3 in later stages of development, we generated a Ppap2b null reporter allele and it was used to establish its expression pattern in E8.5-13.5 embryos. We found that Ppap2b expression during these stages was highly dynamic with significant expression in structures where multiple inductive interactions occur such as the limb buds, mammary gland primordia, heart cushions and valves among others. These observations suggest that Lpp3 expression may play a key role in modulating/integrating multiple signaling pathways during development.
Ppap2b/ lpp3报告基因缺失等位基因的产生及其在胚胎发生过程中的表达。
我们对生物活性脂质在发育过程中如何参与的了解有限,主要是由于脂质研究的困难。这些脂质的可用性是由脂质磷酸酶(LPPs)调节的。编码Lpp3同工酶的Ppap2b的靶向失活具有严重的发育缺陷。由于胚胎外血管缺陷,Lpp3缺陷胚胎在E9.5左右死亡,难以分析其在小鼠发育后期的参与情况。为了获得关于Lpp3可能参与后期发育的一些预测信息,我们生成了一个Ppap2b空报告等位基因,并用于建立其在E8.5-13.5胚胎中的表达模式。我们发现Ppap2b在这些阶段的表达是高度动态的,在肢体芽、乳腺原基、心脏垫和瓣膜等发生多种诱导相互作用的结构中有显著表达。这些观察结果表明,Lpp3的表达可能在发育过程中调节/整合多种信号通路中发挥关键作用。
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