Developmental expression of Apnanos during oogenesis and embryogenesis in the parthenogenetic pea aphid Acyrthosiphon pisum.

Chun‐che Chang, Ting-yu Huang, C. E. Cook, Gee-Way Lin, Chun-Liang Shih, R. Chen
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引用次数: 24

Abstract

Among genes that are preferentially expressed in germ cells, nanos and vasa are the two most conserved germline markers in animals. Both genes are usually expressed in germ cells in the adult gonads, and often also during embryogenesis. Both nanos-first or vasa-first expression patterns have been observed in embryos, implying that the molecular networks governing germline development vary among species. Previously we identified Apvasa, a vasa homologue expressed in germ cells throughout all developmental stages in the parthenogenetic and viviparous pea aphid Acyrthosiphon pisum. In asexual A. pisum, oogenesis is followed by embryogenesis, and both occur within the ovarioles. In order to understand the temporal and spatial distribution of nanos versus vasa during oogenesis and embryogenesis, we isolated a nanos homologue, Apnanos, and studied its expression. In adults, Apnanos is preferentially expressed in the ovaries. In early embryos, Apnanos transcripts are localized to the cytoplasm of cellularizing germ cells, and soon thereafter are restricted to the newly segregated germ cells in the posterior region of the cellularized blastoderm. These results strongly suggest that the Apnanos gene is a germline marker and is involved in germline specification in asexual A. pisum. However, during the middle stages of development, when germline migration occurs, Apnanos is not expressed in the migrating germ cells expressing Apvasa, suggesting that Apnanos is not directly associated with germline migration.
孤雌生殖豌豆蚜虫卵发生和胚胎发生过程中Apnanos的发育表达。
在生殖细胞中优先表达的基因中,nanos和vasa是动物中最保守的两种种系标记。这两种基因通常在成年性腺的生殖细胞中表达,也经常在胚胎发生期间表达。在胚胎中观察到纳米优先或血管优先的表达模式,这意味着控制种系发育的分子网络因物种而异。先前,我们在单性生殖和胎生豌豆蚜虫Acyrthosiphon pisum的所有发育阶段的生殖细胞中都发现了一种异构体vasa。在无性雄蜂中,卵发生之后是胚胎发生,两者都发生在子房内。为了了解纳米和血管在卵发生和胚胎发生过程中的时空分布,我们分离了纳米同源物Apnanos,并对其表达进行了研究。在成人中,Apnanos优先在卵巢中表达。在早期胚胎中,Apnanos转录本定位于细胞化生殖细胞的细胞质中,此后不久被限制在细胞化囊胚后区新分离的生殖细胞中。这些结果有力地表明Apnanos基因是一种生殖系标记,参与了无性蓼的生殖系规范。然而,在发育中期,当种系迁移发生时,Apnanos在表达Apvasa的迁移生殖细胞中不表达,这表明Apnanos与种系迁移没有直接关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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