A phytochemical investigation and evaluation of the antioxidant and antibacterial activities of the stem bark extract of Clematis simensis (Yeazo hareg)

Journal of Chemical Research-s Pub Date : 2022-09-01 DOI:10.1177/17475198221127398

Misganaw T. Ayana, Yekedem G Mehari, B. B. Beyene

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引用次数: 2

Abstract

Medicinal plants have been used for the treatment of various diseases and still play an important role in covering the basic health needs in the developing countries like Ethiopia. In this study, successive extraction, partial purification using column chromatograpy, phytochemical screening, determination of the total phenolic and flavonoid content, and the antioxidant and antibacterial activities of the petroleum ether, chloroform, and ethanol stem bark extracts and fractions of Clematis simensis are reported. The results show the presence of different valuable phytochemicals. The total phenolic content is 54.744, 68.933, and 87.522 mg g–1 of gallic acid equivalent for the petroleum ether, chloroform, and ethanol extracts, respectively. The total flavonoid content of the petroleum ether, chloroform, and ethanol extracts are 22.770, 37.223, and 52.289 mgCE g–1, respectively. This could lead to potential biological activity of the plant extract. Besides, the ethanol extract exhibits higher antioxidant activity than all the tested extracts. Likewise, the extracts have promising antibacterial activity with that of the ethanol extract being higher than those of the chloroform and petroleum extracts as well as isolated fractions.

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铁线莲茎皮提取物抗氧化、抗菌活性的植物化学研究及评价

药用植物已被用于治疗各种疾病，并且在满足埃塞俄比亚等发展中国家的基本保健需求方面仍然发挥着重要作用。本文报道了铁线莲茎皮的连续提取、柱层析部分纯化、植物化学筛选、总酚和类黄酮含量测定、抗氧化和抗菌活性。结果表明，该植物中存在多种有价值的植物化学物质。石油醚、氯仿和乙醇提取物的总酚含量分别为54.744、68.933和87.522 mg g-1(没食子酸当量)。石油醚、氯仿和乙醇提取物的总黄酮含量分别为22.770、37.223和52.289 mgCE g-1。这可能导致植物提取物具有潜在的生物活性。此外，乙醇提取物具有较高的抗氧化活性。同样，该提取物具有良好的抑菌活性，其中乙醇提取物的抑菌活性高于氯仿和石油提取物以及分离馏分。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Chemical Research-s 化学科学, 有机化学, 有机合成

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Chemical Research is a peer reviewed journal that publishes full-length review and research papers in all branches of experimental chemistry. The journal fills a niche by also publishing short papers, a format which favours particular types of work, e.g. the scope of new reagents or methodology, and the elucidation of the structure of novel compounds. Though welcome, short papers should not result in fragmentation of publication, they should describe a completed piece of work. The Journal is not intended as a vehicle for preliminary publications. The work must meet all the normal criteria for acceptance as regards scientific standards. Papers that contain extensive biological results or material relating to other areas of science may be diverted to more appropriate specialist journals. Areas of coverage include: Organic Chemistry; Inorganic Chemistry; Materials Chemistry; Crystallography; Computational Chemistry.