T4 Bacteriophage based Extended Gate Field Effect Transistors (T4B-EGFETs) for Bacteria Detection

Abstract

A T4 Bacteriophage based Extended Gate Field Effect Transistor (T4B-EGFET) was developed for viable bacteria detection. T4 bacteriophages were chemically immobilized on the surface of the extended gate connected to a MOSFET device for sensitive and specific detection of E. coli B bacteria cells. The capturing of the target bacteria cells by the anchored phages will induce a chemical potential, resulting in the shift of the threshold voltage, based on which the limit of detection (LOD) was obtained as 14±3 cfu/mL with a wide dynamic detection range (102~108 cfu/mL). Generalized gm/Id theory was employed for normalized electronic-electrochemical sensitivity analysis and the FET optimized working regime had been identified to be moderate inversion for bacteria detection.