Advanced Fabrication of miRNA-Based Electrochemical Nanobiosensor for Diagnosis of Breast Cancer

Q1 Engineering

Nanomanufacturing and Metrology Pub Date : 2022-09-07 DOI:10.3390/nanomanufacturing2030011

C. I. Kuru, S. Akgöl

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引用次数: 0

Abstract

Early diagnosis is the key to easy, low cost, and effective treatment of breast cancer. Therefore, studies have been accelerated to identify breast cancer diagnostic biomarkers and diagnose cancer before it progresses. The use of miR-155 as a potential biomarker in breast cancer, which has different levels at different stages of the disease, provides a simple serological test for breast cancer prognosis/diagnosis, follow-up, and treatment. Nanopolymers containing different functional groups that are formed by thiol affinity technique were synthesized by mini emulsion polymerization method and advanced characterization studies were carried out in this study to be used as bioactive layers in the nanobiosensor system for miRNA detection. The working conditions of the electrochemical nanobiosensor in which nanopolymers are used as bioactive layers were optimized. Analytical measurement characteristics and validation studies of the nanobiosensor were determined and analysis was performed on commercial blood serum. The potential of the developed electrochemical biosensor to be used as a medical diagnostic kit was explained by comparing it with commercial miRNA kit currently used for the detection of miR-155. This novel nanobiosensor provide sensitive, reliable, and rapid detection of miR-155 and it can provide the potential for breast cancer early diagnosis, prognosis, and follow-up.

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基于mirna的乳腺癌诊断电化学纳米传感器的先进制造

早期诊断是简单、低成本、有效治疗乳腺癌的关键。因此，加快了识别乳腺癌诊断生物标志物和在癌症发展之前进行诊断的研究。miR-155作为乳腺癌的潜在生物标志物，在疾病的不同阶段具有不同的水平，为乳腺癌的预后/诊断、随访和治疗提供了一种简单的血清学检测。采用微乳液聚合的方法合成了巯基亲和技术形成的含不同官能团的纳米聚合物，并对其进行了深入的表征研究，作为纳米生物传感器系统中检测miRNA的生物活性层。优化了以纳米聚合物为生物活性层的电化学纳米生物传感器的工作条件。确定了纳米生物传感器的分析测量特性和验证研究，并在商品血清上进行了分析。通过将所开发的电化学生物传感器与目前用于检测miR-155的商用miRNA试剂盒进行比较，解释了其作为医疗诊断试剂盒的潜力。这种新型纳米生物传感器可以灵敏、可靠、快速地检测miR-155，为乳腺癌的早期诊断、预后和随访提供可能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nanomanufacturing and Metrology Materials Science-Materials Science (miscellaneous)

CiteScore

5.40

自引率

0.00%

发文量

期刊介绍： Nanomanufacturing and Metrology is a peer-reviewed, international and interdisciplinary research journal and is the first journal over the world that provides a principal forum for nano-manufacturing and nano-metrology.Nanomanufacturing and Metrology publishes in the forms including original articles, cutting-edge communications, timely review papers, technical reports, and case studies. Special issues devoted to developments in important topics in nano-manufacturing and metrology will be published periodically.Nanomanufacturing and Metrology publishes articles that focus on, but are not limited to, the following areas:• Nano-manufacturing and metrology• Atomic manufacturing and metrology• Micro-manufacturing and metrology• Physics, chemistry, and materials in micro-manufacturing, nano-manufacturing, and atomic manufacturing• Tools and processes for micro-manufacturing, nano-manufacturing and atomic manufacturing