Successive passaging through an apple host of six low-virulent Neonectria ditissima isolates increased virulence in one of them

Q3 Agricultural and Biological Sciences

New Zealand Plant Protection Pub Date : 2019-07-26 DOI:10.30843/NZPP.2019.72.300

R. Scheper, B. M. Fisher, J. K. Bowen, N. T. Amponsah, D. Hedderley

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引用次数: 2

Abstract

Neonectria ditissima is a serious pathogen of apple. Low-virulent cultures of this fungus have been isolated from cankers, but how and why low-virulent isolates can infect apple is unknown. Rasp wounds on ‘Royal Gala’ trees were inoculated with agar plugs from six low-virulent N. ditissima isolates in a glasshouse. Neonectria ditissima was re-isolated 10 weeks after inoculation. Agar plugs from the re-isolated cultures were used to inoculate ‘Royal Gala’ trees again. After the second re-isolation, conidial suspensions of the original cultures and the 2 x 6 re-isolates were used to determine the virulence on ‘Royal Gala’ compared with field-collected conidia. Three cultures did not cause any lesions; neither did their re-isolates. The re-isolates of two cultures did not differ in virulence compared with the originals. However, the virulence of one isolate increased with each re-isolation, with the second re-isolation causing significantly more lesions, lesion development occurring faster and the lesions being larger than those caused by the original isolate. Therefore, the virulence of N. ditissima isolates can change over time, with loss or gain possibly being attributed to epigenetic and/or genetic changes in the genome.

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连续传代6株低毒力的新树突菌(Neonectria ditissima)在苹果宿主中，增加了其中一株的毒力

苹果新电子虫是一种严重的苹果致病菌。这种真菌的低毒力培养物已经从溃疡病中分离出来，但低毒力分离物如何以及为什么会感染苹果尚不清楚。在一个温室中，用6株低毒N. ditissima分离株的琼脂塞接种“Royal Gala”树的刺伤。接种10周后重新分离新蜱。重新分离培养的琼脂塞用于再次接种' Royal Gala '树。在第二次再分离后，用原培养物的分生孢子悬浮液和2 × 6次再分离的分生孢子与野外收集的分生孢子进行比较，以确定对' Royal Gala '的毒力。三种培养未引起任何病变;它们的再分离也没有。两种培养的再分离株与原菌株的毒力没有差异。然而，随着每次分离，一个分离物的毒力增加，第二次再分离引起的病变明显更多，病变发展更快，病变更大。因此，ditissima分离株的毒力可以随着时间的推移而改变，其损失或增加可能归因于基因组的表观遗传和/或遗传变化。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

New Zealand Plant Protection Agricultural and Biological Sciences-Insect Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： New Zealand Plant Protection is the journal of the New Zealand Plant Protection Society. It publishes original research papers on all aspects of biology, ecology and control of weeds, vertebrate and invertebrate pests, and pathogens and beneficial micro-organisms in agriculture, horticulture, forestry and natural ecosystems of relevance to New Zealand.